Details

Autor(en) / Beteiligte

• Wang, L. M.
• Wen, J. X.
• Yuan, J. L.
• Cang, Ming
• Liu, D. J.

Titel

Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development

Ist Teil von

• Cytotechnology (Dordrecht), 2012-03, Vol.64 (2), p.165-172

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2012

Quelle

SpringerLink

Beschreibungen/Notizen

• This study aimed to assess the efficiency and effects of insulin-like growth factor receptor-1 (IGF-IR) siRNA knockdown during bovine preimplantation embryonic development. In oocytes injected with IGF-IR siRNA, the relative IGF-IR mRNA levels compared to controls were 28% and 46% at 6 and 24 h after injection, respectively. With respect to the injection of IGF-IR siRNA in zygotes, 24 h after injection the relative levels of IGF-IR mRNA and protein in the two-cell embryos were 74% and 78% of those in the controls, respectively. IGF-IR siRNA reduced blastocyst formation (23.2%) compared to siRNA controls (33.0%) and uninjected oocytes (35.4%; P  < 0.05) and the number of viable cells per IGF-IR siRNA-treated blastocyst (64 ± 3) was significantly reduced, compared to control siRNA and uninjected blastocysts (81 ± 3 and 116 ± 4; P  < 0.01). In conclusion, IGF-IR siRNA knockdown reduces the development of bovine embryos, and microinjection in zygotes can decrease blastocyst cell number.