Details

Autor(en) / Beteiligte

• Brissette-Storkus, Cynthia S
• Reynolds, Stephanie M
• Lepisto, Andrew J
• Hendricks, Robert L

Titel

Identification of a Novel Macrophage Population in the Normal Mouse Corneal Stroma

Ist Teil von

• Investigative ophthalmology & visual science, 2002-07, Vol.43 (7), p.2264-2271

Ort / Verlag

Rockville, MD: ARVO

Erscheinungsjahr

2002

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• To examine the normal murine corneal stroma for the presence of bone marrow-derived leukocytes. Wholemounts of paraformaldehyde-fixed corneal stroma from normal mice at 5 to 16 weeks of age were examined in single- and double-color immunomorphologic studies performed with confocal microscopy. The phenotype, morphology, distribution, and density of immunopositive cells were determined. Numerous CD45(+) cells with pleomorphic and dendriform morphology were found within the pericentral and central region of the corneal stroma (200-300 cells/mm(2)). Dual-color immunostaining demonstrated that 100% of the CD45(+) cells coexpressed CD11b and 50% coexpressed F4/80. Approximately 30% of the total cells and 50% of the F4/80(+) cells coexpressed major histocompatibility complex (MHC) class II antigens. Very small to negligible numbers of cells expressed markers of dendritic cells (CD11c) or granulocytes (Ly6G). Markers for T-cells and NK cells were absent from the corneal stroma, indicating that all the cells identified in the stroma were of the myeloid lineage. The normal murine corneal stroma contains a significant number of CD45(+) leukocytes. Most these cells express the CD11b marker, but not other dendrite, granulocyte, T-cell, or NK markers, placing them in the monocyte/macrophage lineage.