Cytometry. Part A, 2011-11, Vol.79A (11), p.897-902
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Induction of DNA damage signaling by oxidative stress in relation to DNA replication as detected using “click chemistry”
- Ist Teil von
- Cytometry. Part A, 2011-11, Vol.79A (11), p.897-902
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2011
- Quelle
- Wiley Online Library - AutoHoldings Journals
- Beschreibungen/Notizen
- Induction of DNA damage by oxidants such as H2O2 activates the complex network of DNA damage response (DDR) pathways present in cells to initiate DNA repair, halt cell cycle progression, and prepare an apoptotic reaction. We have previously reported that activation of Ataxia Telangiectasia Mutated protein kinase (ATM) and induction of γH2AX are among the early events of the DDR induced by exposure of cells to H2O2, and in human pulmonary carcinoma A549 cells, both events were expressed predominantly during S‐phase. This study was designed to further explore a correlation between these events and DNA replication. Toward this end, we utilized 5‐ethynyl‐2′deoxyuridine (EdU) and the “click chemistry” approach to label DNA during replication, followed by exposure of A549 cells to H2O2. Multiparameter laser scanning cytometric analysis of these cells made it possible to identify DNA replicating cells and directly correlate H2O2‐induced ATM activation and induction of γH2AX with DNA replication on a cell by cell basis. After pulse‐labeling with EdU and exposure to H2O2, confocal microscopy was also used to examine the localization of DNA replication sites (“replication factories”) versus the H2AX phosphorylation sites (γH2AX foci) in nuclear chromatin in an attempt to observe the absence or presence of colocalization. The data indicate a close association between DNA replication and H2AX phosphorylation in A549 cells, suggesting that these DNA damage response events may be triggered by stalled replication forks and perhaps also by induction of DNA double‐strand breaks at the primary DNA lesions induced by H2O2. © 2011 International Society for Advancement of Cytometry.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1552-4922, 1552-4930eISSN: 1552-4930DOI: 10.1002/cyto.a.21137Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3238684
- Format
- –
- Schlagworte
- Apoptosis, Apoptosis - drug effects, Ataxia telangiectasia mutated protein, Ataxia Telangiectasia Mutated Proteins, Carcinoma, Cell cycle, Cell Cycle - drug effects, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Cell Line, Tumor, Chromatin, Click Chemistry - methods, Confocal microscopy, Cytometry, Data processing, Deoxyuridine - analogs & derivatives, Deoxyuridine - metabolism, DNA biosynthesis, DNA Breaks, Double-Stranded - drug effects, DNA damage, DNA damage response, DNA Repair, DNA Replication - drug effects, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, EdU incorporation, Epithelial Cells - cytology, Epithelial Cells - drug effects, Epithelial Cells - metabolism, Gene Expression, Histones - genetics, Histones - metabolism, Humans, Hydrogen peroxide, Hydrogen Peroxide - adverse effects, Laser Scanning Cytometry - methods, Lung carcinoma, Microscopy, Confocal, Oxidants, Oxidative Stress, Phosphorylation, Phosphorylation - drug effects, Protein-Serine-Threonine Kinases - genetics, Protein-Serine-Threonine Kinases - metabolism, reactive oxygen species, Replication, Replication forks, replication stress, S phase, Signal transduction, Signal Transduction - drug effects, Single-Cell Analysis - methods, Staining and Labeling - methods, Tumor Suppressor Proteins - genetics, Tumor Suppressor Proteins - metabolism