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BibTeX
Neuronal activity regulates glutamate transporter dynamics in developing astrocytes
Glia, 2012-02, Vol.60 (2), p.175-188
Benediktsson, Adrienne M.
Marrs, Glen S.
Tu, Jian Cheng
Worley, Paul F.
Rothstein, Jeffrey D.
Bergles, Dwight E.
Dailey, Michael E.
2012
Details
Autor(en) / Beteiligte
Benediktsson, Adrienne M.
Marrs, Glen S.
Tu, Jian Cheng
Worley, Paul F.
Rothstein, Jeffrey D.
Bergles, Dwight E.
Dailey, Michael E.
Titel
Neuronal activity regulates glutamate transporter dynamics in developing astrocytes
Ist Teil von
Glia, 2012-02, Vol.60 (2), p.175-188
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2012
Link zum Volltext
Quelle
Wiley Blackwell Single Titles
Beschreibungen/Notizen
Glutamate transporters (GluTs) maintain a low ambient level of glutamate in the central nervous system (CNS) and shape the activation of glutamate receptors at synapses. Nevertheless, the mechanisms that regulate the trafficking and localization of transporters near sites of glutamate release are poorly understood. Here, we examined the subcellular distribution and dynamic remodeling of the predominant GluT GLT‐1 (excitatory amino acid transporter 2, EAAT2) in developing hippocampal astrocytes. Immunolabeling revealed that endogenous GLT‐1 is concentrated into discrete clusters along branches of developing astrocytes that were apposed preferentially to synapsin‐1 positive synapses. Green fluorescent protein (GFP)‐GLT‐1 fusion proteins expressed in astrocytes also formed distinct clusters that lined the edges of astrocyte processes, as well as the tips of filopodia and spine‐like structures. Time‐lapse three‐dimensional confocal imaging in tissue slices revealed that GFP‐GLT‐1 clusters were dynamically remodeled on a timescale of minutes. Some transporter clusters moved within developing astrocyte branches as filopodia extended and retracted, while others maintained stable positions at the tips of spine‐like structures. Blockade of neuronal activity with tetrodotoxin reduced both the density and perisynaptic localization of GLT‐1 clusters. Conversely, enhancement of neuronal activity increased the size of GLT‐1 clusters and their proximity to synapses. Together, these findings indicate that neuronal activity influences both the organization of GluTs in developing astrocyte membranes and their position relative to synapses. © 2011 Wiley Periodicals, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0894-1491
eISSN: 1098-1136
DOI: 10.1002/glia.21249
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3232333
Format
–
Schlagworte
activity
,
Animals
,
Animals, Newborn
,
astrocyte
,
Astrocytes - cytology
,
Astrocytes - metabolism
,
biolistics
,
Cell Differentiation - physiology
,
EAAT2
,
Excitatory Amino Acid Transporter 2 - metabolism
,
GLT-1
,
Hippocampus - cytology
,
Hippocampus - growth & development
,
Neurons - cytology
,
Neurons - metabolism
,
Organ Culture Techniques
,
Rats
,
Rats, Sprague-Dawley
,
Synapses - metabolism
,
time-lapse
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