The Plant cell, 2011-10, Vol.23 (10), p.3696-3710
2011
Details
- Autor(en) / Beteiligte
- Titel
- Actin Interacting Protein1 and Actin Depolymerizing Factor Drive Rapid Actin Dynamics in Physcomitrella patens
- Ist Teil von
- The Plant cell, 2011-10, Vol.23 (10), p.3696-3710
- Ort / Verlag
- United States: American Society of Plant Biologists
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The remodeling of actin networks is required for a variety of cellular processes in eukaryotes. In plants, several actin binding proteins have been implicated in remodeling cortical actin filaments (F-actin). However, the extent to which these proteins support F-actin dynamics in planta has not been tested. Using reverse genetics, complementation analyses, and cell biological approaches, we assessed the in vivo function of two actin turnover proteins: actin interacting proteini (AIP1) and actin depolymerizing factor (ADF). We report that AIP1 is a single-copy gene in the moss Physcomitrella patens. AIP1 knockout plants are viable but have reduced expansion of tip-growing cells. AIP1 is diffusely cytosolic and functions in a common genetic pathway with ADF to promote tip growth. Specifically, ADF can partially compensate for loss of AIP1, and AIP1 requires ADF for function. Consistent with a role in actin remodeling, AIP1 knockout lines accumulate F-actin bundles, have fewer dynamic ends, and have reduced severing frequency. Importantly, we demonstrate that AIP1 promotes and ADF is essential for cortical F-actin dynamics.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1040-4651eISSN: 1532-298XDOI: 10.1105/tpc.111.090753Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3229144
- Format
- –
- Schlagworte
- Actin, Actin Cytoskeleton - metabolism, Actin Cytoskeleton - ultrastructure, Actin depolymerizing factors, Actin Depolymerizing Factors - genetics, Actin Depolymerizing Factors - metabolism, Actins, Actins - metabolism, Base Sequence, Binding proteins, Bryopsida - genetics, Bryopsida - growth & development, Bryopsida - metabolism, Bryopsida - ultrastructure, Cell growth, Cell walls, Cytosol - metabolism, DNA, Plant - chemistry, DNA, Plant - genetics, Gene Knockout Techniques, Genetic Complementation Test, Imaging, Microfilament Proteins - genetics, Microfilament Proteins - metabolism, Microfilaments, Molecular Sequence Data, Moss, Mosses, Physiological aspects, Plant cells, Plant growth, Plant physiology, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Roots - genetics, Plant Roots - growth & development, Plant Roots - metabolism, Plant Roots - ultrastructure, Plants, Plants, Genetically Modified, Reverse Genetics, RNA, Plant - genetics, Sequence Analysis, DNA, Time Factors