Details

Autor(en) / Beteiligte

• Rackham, Oliver
• Shearwood, Anne-Marie J
• Mercer, Tim R
• Davies, Stefan M K
• Mattick, John S
• Filipovska, Aleksandra

Titel

Long noncoding RNAs are generated from the mitochondrial genome and regulated by nuclear-encoded proteins

Ist Teil von

• RNA (Cambridge), 2011-12, Vol.17 (12), p.2085-2093

Ort / Verlag

United States: Cold Spring Harbor Laboratory Press

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Human mitochondrial long noncoding RNAs (lncRNAs) have not been described to date. By analysis of deep-sequencing data we have identified three lncRNAs generated from the mitochondrial genome and confirmed their expression by Northern blotting and strand-specific qRT-PCR. We show that the abundance of these lncRNAs is comparable to their complementary mRNAs and that nuclear-encoded mitochondrial proteins involved in RNA processing regulate their expression. We also identify the 5' and 3' transcript ends of the three lncRNAs and show that mitochondrial RNase P protein 1 (MRPP1) is important for the processing of these transcripts. Finally, we show that mitochondrial lncRNAs form intermolecular duplexes and that their abundance is cell- and tissue-specific, suggesting a functional role in the regulation of mitochondrial gene expression.