Details

Autor(en) / Beteiligte

• Smith, David W.E.
• McNamara, Anne L.
• Vold, Barbara S.

Titel

Lysine tRNAs from Bacillus subtilis 168: function of the isoacceptors in a rabbit reticulocyte cell-free protein-synthesizing system

Ist Teil von

• Nucleic acids research, 1982-05, Vol.10 (10), p.3117-3123

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

1982

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The two principal tRNALys isoaccepting species of Bacillus subtilis were compared in their functional activity in translating rabbit globin. Although neither species demonstrates any preference in reading either of the lysine codons, there is an overall preference for tRNA3Lys in lysine incorporation. The ratios of lysine incorporated by the two species into the different lysine-containing sites in the globin subunits vary over a more than two-fold range. As described in the accompanying paper, tRNA1Lys is a hypomodified form of tRNA3Lys. Consistent with studies on other tRNA species, the fully modified isoacceptor functions preferentially. In contrast to these results, however, the fully modified isoacceptor (tRNA3Lys) is found predominantly in rapidly dividing cells while the hypomodified isoacceptor (tRNA1Lys) predominates in the stationary cells and spores of B. subtilis.