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Complete Sequencing of the bla NDM-1-Positive IncA/C Plasmid from Escherichia coli ST38 Isolate Suggests a Possible Origin from Plant Pathogens
Ist Teil von
PloS one, 2011-09, Vol.6 (9)
Ort / Verlag
San Francisco, USA: Public Library of Science
Erscheinungsjahr
2011
Link zum Volltext
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
The complete sequence of the plasmid pNDM-1_Dok01 carrying New Delhi metallo-β-lactamase (NDM-1) was determined by whole genome shotgun sequencing using
Escherichia coli
strain NDM-1_Dok01 (multilocus sequence typing type: ST38) and the transconjugant
E. coli
DH10B. The plasmid is an IncA/C incompatibility type composed of 225 predicted coding sequences in 195.5 kb and partially shares a sequence with
bla
CMY-2
-positive IncA/C plasmids such as
E. coli
AR060302 pAR060302 (166.5 kb) and
Salmonella enterica
serovar Newport pSN254 (176.4 kb). The
bla
NDM-1
gene in pNDM-1_Dok01 is terminally flanked by two IS
903
elements that are distinct from those of the other characterized NDM-1 plasmids, suggesting that the
bla
NDM-1
gene has been broadly transposed, together with various mobile elements, as a cassette gene. The chaperonin
groES
and
groEL
genes were identified in the
bla
NDM-1
-related composite transposon, and phylogenetic analysis and guanine-cytosine content (GC) percentage showed similarities to the homologs of plant pathogens such as
Pseudoxanthomonas
and
Xanthomonas
spp., implying that plant pathogens are the potential source of the
bla
NDM-1
gene. The complete sequence of pNDM-1_Dok01 suggests that the
bla
NDM-1
gene was acquired by a novel composite transposon on an extensively disseminated IncA/C plasmid and transferred to the
E. coli
ST38 isolate.