Genome-wide Analyses of Transcription Factor GATA3-Mediated Gene Regulation in Distinct T Cell Types
2011
Details
- Autor(en) / Beteiligte
- Titel
- Genome-wide Analyses of Transcription Factor GATA3-Mediated Gene Regulation in Distinct T Cell Types
- Ist Teil von
- Immunity (Cambridge, Mass.), 2011-08, Vol.35 (2), p.299-311
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The transcription factor GATA3 plays an essential role during T cell development and T helper 2 (Th2) cell differentiation. To understand GATA3-mediated gene regulation, we identified genome-wide GATA3 binding sites in ten well-defined developmental and effector T lymphocyte lineages. In the thymus, GATA3 directly regulated many critical factors, including Th-POK, Notch1, and T cell receptor subunits. In the periphery, GATA3 induced a large number of Th2 cell-specific as well as Th2 cell-nonspecific genes, including several transcription factors. Our data also indicate that GATA3 regulates both active and repressive histone modifications of many target genes at their regulatory elements near GATA3 binding sites. Overall, although GATA3 binding exhibited both shared and cell-specific patterns among various T cell lineages, many genes were either positively or negatively regulated by GATA3 in a cell type-specific manner, suggesting that GATA3-mediated gene regulation depends strongly on cofactors existing in different T cells. ► GATA3 binding sites are identified in ten developmental and effector T cell lineages ► GATA3 binding has shared and cell-specific patterns among various T cell lineages ► Regulation of gene expression by GATA3 is cell type specific ► GATA3 regulates both active and repressive histone modifications at target enhancers
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1074-7613eISSN: 1097-4180DOI: 10.1016/j.immuni.2011.08.007Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3169184
- Format
- –
- Schlagworte
- Animals, Binding sites, Cell Lineage - genetics, DNA Methylation, Experiments, GATA3 Transcription Factor - genetics, GATA3 Transcription Factor - immunology, GATA3 Transcription Factor - metabolism, Gene expression, Gene Expression Regulation, Genome - immunology, Genome-Wide Association Study, Genomes, Histones - genetics, Histones - metabolism, Lymphopoiesis - genetics, Mice, Mice, Inbred C57BL, Mice, Transgenic, Mutant Proteins - genetics, Mutant Proteins - immunology, Mutant Proteins - metabolism, Protein Binding, Receptor, Notch1 - genetics, Receptor, Notch1 - metabolism, Receptors, Antigen, T-Cell, alpha-beta - genetics, Receptors, Antigen, T-Cell, alpha-beta - metabolism, Rodents, Studies, T-Lymphocyte Subsets - immunology, T-Lymphocyte Subsets - metabolism, T-Lymphocyte Subsets - pathology, Th2 Cells - immunology, Th2 Cells - metabolism, Th2 Cells - pathology, Transcription factors, Transcription Factors - genetics, Transcription Factors - metabolism