Cell, 2011-06, Vol.145 (6), p.969-980
2011
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- Autor(en) / Beteiligte
- Titel
- Riboneogenesis in Yeast
- Ist Teil von
- Cell, 2011-06, Vol.145 (6), p.969-980
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2011
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- Glucose is catabolized in yeast via two fundamental routes, glycolysis and the oxidative pentose phosphate pathway, which produces NADPH and the essential nucleotide component ribose-5-phosphate. Here, we describe riboneogenesis, a thermodynamically driven pathway that converts glycolytic intermediates into ribose-5-phosphate without production of NADPH. Riboneogenesis begins with synthesis, by the combined action of transketolase and aldolase, of the seven-carbon bisphosphorylated sugar sedoheptulose-1,7-bisphosphate. In the pathway's committed step, sedoheptulose bisphosphate is hydrolyzed to sedoheptulose-7-phosphate by the enzyme sedoheptulose-1,7-bisphosphatase (SHB17), whose activity we identified based on metabolomic analysis of the corresponding knockout strain. The crystal structure of Shb17 in complex with sedoheptulose-1,7-bisphosphate reveals that the substrate binds in the closed furan form in the active site. Sedoheptulose-7-phosphate is ultimately converted by known enzymes of the nonoxidative pentose phosphate pathway to ribose-5-phosphate. Flux through SHB17 increases when ribose demand is high relative to demand for NADPH, including during ribosome biogenesis in metabolically synchronized yeast cells. [Display omitted] ► Riboneogenesis converts three-carbon glycolytic intermediates into ribose ► Sedoheptulose-1,7-bisphosphate is a key intermediate in riboneogenesis in yeast ► A sedoheptulose bisphosphatase, Shb17, thermodynamically drives ribose biosynthesis ► Flux through Shb17 is high when demand for ribose exceeds that for reducing power
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0092-8674eISSN: 1097-4172DOI: 10.1016/j.cell.2011.05.022Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3163394
- Format
- –
- Schlagworte
- active sites, biogenesis, Biosynthetic Pathways, crystal structure, Crystallography, X-Ray, fructose-bisphosphate aldolase, Gene Deletion, glucose, glycolysis, metabolomics, Models, Molecular, NADP (coenzyme), pentose phosphate cycle, Pentose Phosphate Pathway, Phosphoric Monoester Hydrolases - chemistry, Phosphoric Monoester Hydrolases - genetics, Phosphoric Monoester Hydrolases - metabolism, ribose, Ribosemonophosphates - biosynthesis, ribosomes, Saccharomyces cerevisiae - enzymology, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, thermodynamics, transketolase, yeasts