Details

Autor(en) / Beteiligte

• Kim, Hon-Song
• Murakami, Ryoko
• Quintin, Sophie
• Mori, Masataka
• Ohkura, Kiyotaka
• Tamai, Katsuyuki K
• Labouesse, Michel
• Sakamoto, Hiroshi
• Nishiwaki, Kiyoji

Titel

VAB-10 spectraplakin acts in cell and nuclear migration in Caenorhabditis elegans

Ist Teil von

• Development (Cambridge), 2011-09, Vol.138 (18), p.4013-4023

Ort / Verlag

England: Company of Biologists

Erscheinungsjahr

2011

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Cytoskeletal regulation is important in cell migration. The Caenorhabditis elegans gonadal distal tip cells (DTCs) offer a simple model with which to investigate the mechanism of cell migration in organogenesis. Here, we report that one of the spectraplakin isoforms, VAB-10B1, plays an essential role in cell and nuclear migration of DTCs by regulating the actin and microtubule (MT) cytoskeleton. In the vab-10(tk27) mutant, which lacks VAB-10B1, alignment of filamentous (F)-actin and MTs was weakly and severely disorganized, respectively, which resulted in a failure to translocate the DTC nucleus and a premature termination of DTC migration. An MT growing-tip marker, EBP-2-GFP, revealed that polarized outgrowth of MTs towards the nuclei of migrating DTCs was strikingly impaired in tk27 animals. A vab-10 mini-gene encoding only the actin- and MT-binding domains significantly rescued the gonadal defects, suggesting that VAB-10B1 has a role in linking actin and MT filaments. These results suggest that VAB-10B1/spectraplakin regulates the polarized alignment of MTs, possibly by linking F-actin and MTs, which enables normal nuclear translocation and cell migration of DTCs.