Details

Autor(en) / Beteiligte

• Ewald, Friederike
• Anfinrud, Philip A
• Cammarata, Marco
• Cupane, Antonio
• Wulff, Michael
• Levantino, Matteo
• Ihee, Hyotcherl
• Schotte, Friedrich
• Choi, Jungkweon

Titel

Tracking the structural dynamics of proteins in solution using time-resolved wide-angle X-ray scattering

Ist Teil von

• Nature methods, 2008-10, Vol.5 (10), p.881-886

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• We demonstrate tracking of protein structural changes with time-resolved wide-angle X-ray scattering (TR-WAXS) with nanosecond time resolution. We investigated the tertiary and quaternary conformational changes of human hemoglobin under nearly physiological conditions triggered by laser-induced ligand photolysis. We also report data on optically induced tertiary relaxations of myoglobin and refolding of cytochrome c to illustrate the wide applicability of the technique. By providing insights into the structural dynamics of proteins functioning in their natural environment, TR-WAXS complements and extends results obtained with time-resolved optical spectroscopy and X-ray crystallography.