Details

Autor(en) / Beteiligte

• Biddie, Simon C.
• John, Sam
• Sabo, Pete J.
• Thurman, Robert E.
• Johnson, Thomas A.
• Schiltz, R. Louis
• Miranda, Tina B.
• Sung, Myong-Hee
• Trump, Saskia
• Lightman, Stafford L.
• Vinson, Charles
• Stamatoyannopoulos, John A.
• Hager, Gordon L.

Titel

Transcription Factor AP1 Potentiates Chromatin Accessibility and Glucocorticoid Receptor Binding

Ist Teil von

• Molecular cell, 2011-07, Vol.43 (1), p.145-155

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2011

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Ligand-dependent transcription by the nuclear receptor glucocorticoid receptor (GR) is mediated by interactions with coregulators. The role of these interactions in determining selective binding of GR to regulatory elements remains unclear. Recent findings indicate that a large fraction of genomic GR binding coincides with chromatin that is accessible prior to hormone treatment, suggesting that receptor binding is dictated by proteins that maintain chromatin in an open state. Combining DNaseI accessibility and chromatin immunoprecipitation with high-throughput sequencing, we identify the activator protein 1 (AP1) as a major partner for productive GR-chromatin interactions. AP1 is critical for GR-regulated transcription and recruitment to co-occupied regulatory elements, illustrating an extensive AP1-GR interaction network. Importantly, the maintenance of baseline chromatin accessibility facilitates GR recruitment and is dependent on AP1 binding. We propose a model in which the basal occupancy of transcription factors acts to prime chromatin and direct inducible transcription factors to select regions in the genome. [Display omitted] ► AP1 and GR binding are universally associated with open chromatin ► GR binding is substantially associated with AP1 occupancy ► AP1 binding maintains chromatin accessibility at regulatory elements genome-wide ► AP1 establishes genomic binding patterns for signal-dependent factors such as GR