Details

Autor(en) / Beteiligte

• Yamamoto, M
• Meno, C
• Sakai, Y
• Shiratori, H
• Mochida, K
• Ikawa, Y
• Saijoh, Y
• Hamada, H

Titel

The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse

Ist Teil von

• Genes & development, 2001-05, Vol.15 (10), p.1242-1256

Ort / Verlag

United States: Cold Spring Harbor Laboratory Press

Erscheinungsjahr

2001

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• FoxH1 (FAST) is a transcription factor that mediates signaling by transforming growth factor-beta, Activin, and Nodal. The role of FoxH1 in development has now been investigated by the generation and analysis of FoxH1-deficient (FoxH1(-/-)) mice. The FoxH1(-/-) embryos showed various patterning defects that recapitulate most of the defects induced by the loss of Nodal signaling. A substantial proportion of FoxH1(-/-) embryos failed to orient the anterior-posterior (A-P) axis correctly, as do mice lacking Cripto, a coreceptor for Nodal. In less severely affected FoxH1(-/-) embryos, A-P polarity was established, but the primitive streak failed to elongate, resulting in the lack of a definitive node and its derivatives. Heterozygosity for nodal renders the FoxH1(-/-) phenotype more severe, indicative of a genetic interaction between FoxH1 and nodal. The expression of FoxH1 in the primitive endoderm rescued the A-P patterning defects, but not the midline defects, of FoxH1(-/-) mice. These results indicate that a Nodal-FoxH1 signaling pathway plays a central role in A-P patterning and node formation in the mouse.