Details

Autor(en) / Beteiligte

• Zhao, Bao-Cheng
• Wang, Zhen-Jun
• Mao, Wei-Zheng
• Ma, Hua-Chong
• Han, Jia-Gang
• Zhao, Bo
• Xu, Hui-Min

Titel

CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma

Ist Teil von

• World journal of gastroenterology : WJG, 2011-05, Vol.17 (19), p.2389-2396

Ort / Verlag

United States: Baishideng Publishing Group Co., Limited

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• AIM：To investigate the role of CXC chemokine receptor-4 （CXCR4） and stromal cell-derived factor-1 （SDF-1） in lymph node metastasis of gastric carcinoma.METHODS：In 40 cases of gastric cancer,expression of CXCR4 mRNA in cancer and normal mucous membrane and SDF-1 mRNA in lymph nodes around the stomach was detected using quantitative polymerase chain reaction （PCR） （TaqMan） and immunohistochemistric assay.SGC-7901 and MGC80-3 cancer cells were used to investigate the effect of SDF-1 on cell proliferation and migration.RESULTS：Quantitative reverse transcription PCR and immunohistochemistry revealed that the expression level of CXCR4 in gastric cancer was significantly higher than that in normal mucous membrane （1.6244 ± 1.3801 vs 1.0715 ± 0.5243,P 0.05）.The expression level of CXCR4 mRNA in gastric cancer with lymph node metastasis was also significantly higher than that without lymph node metastasis （0.823 ± 0.551 vs 0.392 ± 0.338,P 0.05）.CXCR4 expression was significantly related to poorly differentiated,high tumor stage and lymph node metastasis.Significant differences in the expression level of SDF-1 mRNA were found between lymph nodes in metastatic gastric cancer and normal nodes （0.5432 ± 0.4907 vs 0.2640 ± 0.2601,P 0.05）.The positive expression of SDF-1 mRNA in lymph nodes of metastatic gastric cancer was consistent with the positive expression of CXCR4 mRNA in gastric cancer （r=0.776,P 0.01）.Additionally,human gastric cancer cell lines expressed CXCR4 and showed vigorous proliferation and migratory responses to SDF-1.AMD3100 （a specific CXCR4 antagonist） was also found to effectively reduce the migration of gastric cancer cells.CONCLUSION：The CXCR4/SDF-1 axis is involved in the lymph node metastasis of gastric cancer.CXCR4 is considered as a potential therapeutic target in the treatment of gastric cancer.