Journal of biotechnology, 2011-05, Vol.153 (3), p.125-132
2011
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- Autor(en) / Beteiligte
- Titel
- Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli
- Ist Teil von
- Journal of biotechnology, 2011-05, Vol.153 (3), p.125-132
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Quantum dots (QDs) were prepared in genetically engineered Escherichia coli ( E. coli) through the introduction of foreign genes encoding a CdS binding peptide. The CdS QDs were successfully separated from the bacteria through two methods, lysis and freezing–thawing of cells, and purified with an anion-exchange resin. High-resolution transmission electron microscopy, X-ray diffraction, luminescence spectroscopy, and energy dispersive X-ray spectroscopy were applied to characterize the as-prepared CdS QDs. The effects of reactant concentrations, bacteria incubation times, and reaction times on QD growth were systematically investigated. Our work demonstrates that genetically engineered bacteria can be used to synthesize QDs. The biologically synthesized QDs are expected to be more biocompatible probes in bio-labeling and imaging.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0168-1656eISSN: 1873-4863DOI: 10.1016/j.jbiotec.2011.03.014Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3102602
- Format
- –
- Schlagworte
- anion exchange, bacteria, Biological and medical sciences, Bioreactors - microbiology, Biosynthesis, Biotechnology, Biotechnology - methods, Cadmium Compounds - chemistry, CdS, Electrophoresis, Polyacrylamide Gel, energy, Escherichia coli, Escherichia coli - chemistry, Escherichia coli - genetics, Escherichia coli - metabolism, freeze-thaw cycles, Fundamental and applied biological sciences. Psychology, genes, Genetic engineering, Genetic Engineering - methods, genetically engineered microorganisms, image analysis, luminescence, Microscopy, Electron, Transmission, Peptides - chemistry, Peptides - genetics, Peptides - metabolism, Quantum Dots, Spectrometry, Fluorescence, spectroscopy, Sulfides - chemistry, transmission electron microscopy, X-radiation, X-ray diffraction