The Journal of cell biology, 2010-09, Vol.190 (6), p.1079-1091
2010
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Exosome release of β-catenin: a novel mechanism that antagonizes Wnt signaling
- Ist Teil von
- The Journal of cell biology, 2010-09, Vol.190 (6), p.1079-1091
- Ort / Verlag
- United States: The Rockefeller University Press
- Erscheinungsjahr
- 2010
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- CD82 and CD9 are tetraspanin membrane proteins that can function as suppressors of tumor metastasis. Expression of CD9 and CD82 in transfected cells strongly suppresses β-catenin-mediated Wnt signaling activity and induces a significant decrease in β-catenin protein levels. Inhibition of Wnt/β-catenin signaling is independent of glycogen synthase kinase-3β and of the proteasome- and lysosome-mediated protein degradation pathways. CD82 and CD9 expression induces β-catenin export via exosomes, which is blocked by a sphingomyelinase inhibitor, GW4869. CD82 fails to induce exosome release of β-catenin in cells that express low levels of E-cadherin. Exosome release from dendritic cells generated from CD9 knockout mice is reduced compared with that from wild-type dendritic cells. These results suggest that CD82 and CD9 down-regulate the Wnt signaling pathway through the exosomal discharge of β-catenin. Thus, exosomal packaging and release of cytosolic proteins can modulate the activity of cellular signaling pathways.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9525eISSN: 1540-8140DOI: 10.1083/jcb.201002049Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3101591
- Format
- –
- Schlagworte
- Animals, Antibodies, Antigens, CD, Antigens, CD - metabolism, Antigens, CD82, Antigens, CD9, beta Catenin, beta Catenin - secretion, Cadherins, Cadherins - metabolism, Cell Line, Cell membranes, CHO cells, Dendritic cells, Exosomes, Exosomes - secretion, Exosomes - ultrastructure, Gene Knockdown Techniques, Genes, Reporter, Genes, Reporter - genetics, Glycogen Synthase Kinase 3, Glycogen Synthase Kinase 3 - metabolism, Glycogen Synthase Kinase 3 beta, HeLa cells, Humans, Immunology, Kangai-1 Protein - metabolism, Life Sciences, Lysosomes, Lysosomes - metabolism, Membrane Glycoproteins, Membrane Glycoproteins - metabolism, Mice, Models, Biological, Plasmids, Proteasome Endopeptidase Complex, Proteasome Endopeptidase Complex - metabolism, Protein Processing, Post-Translational, Protein Stability, Protein Transport, Signal Transduction, Tetraspanin-29, Transfection, Western blotting, Wnt Proteins, Wnt Proteins - metabolism