Details

Autor(en) / Beteiligte

• Kaneko, Hiroki
• Dridi, Sami
• Tarallo, Valeria
• Gelfand, Bradley D.
• Fowler, Benjamin J.
• Cho, Won Gil
• Kleinman, Mark E.
• Ponicsan, Steven L.
• Hauswirth, William W.
• Chiodo, Vince A.
• Karikó, Katalin
• Yoo, Jae Wook
• Lee, Dong-ki
• Hadziahmetovic, Majda
• Song, Ying
• Misra, Smita
• Chaudhuri, Gautam
• Buaas, Frank W.
• Braun, Robert E.
• Hinton, David R.
• Zhang, Qing
• Grossniklaus, Hans E.
• Provis, Jan M.
• Madigan, Michele C.
• Milam, Ann H.
• Justice, Nikki L.
• Albuquerque, Romulo J. C.
• Blandford, Alexander D.
• Bogdanovich, Sasha
• Hirano, Yoshio
• Witta, Jassir
• Fuchs, Elaine
• Littman, Dan R.
• Ambati, Balamurali K.
• Rudin, Charles M.
• Chong, Mark M. W.
• Provost, Patrick
• Kugel, Jennifer F.
• Goodrich, James A.
• Dunaief, Joshua L.
• Baffi, Judit Z.
• Ambati, Jayakrishna

Titel

DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration

Ist Teil von

• Nature (London), 2011-03, Vol.471 (7338), p.325-330

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2011

Quelle

Psychology and Behavioral Sciences Collection

Beschreibungen/Notizen

• Geographic atrophy (GA), an untreatable advanced form of age-related macular degeneration, results from retinal pigmented epithelium (RPE) cell degeneration. Here we show that the microRNA (miRNA)-processing enzyme DICER1 is reduced in the RPE of humans with GA, and that conditional ablation of Dicer1 , but not seven other miRNA-processing enzymes, induces RPE degeneration in mice. DICER1 knockdown induces accumulation of Alu RNA in human RPE cells and Alu -like B1 and B2 RNAs in mouse RPE. Alu RNA is increased in the RPE of humans with GA, and this pathogenic RNA induces human RPE cytotoxicity and RPE degeneration in mice. Antisense oligonucleotides targeting Alu /B1/B2 RNAs prevent DICER1 depletion-induced RPE degeneration despite global miRNA downregulation. DICER1 degrades Alu RNA, and this digested Alu RNA cannot induce RPE degeneration in mice. These findings reveal a miRNA-independent cell survival function for DICER1 involving retrotransposon transcript degradation, show that Alu RNA can directly cause human pathology, and identify new targets for a major cause of blindness. Blindness linked to DICER1 activity Kaneko et al . report that geographic atrophy, a form of age-related blindness caused by cell death in the retinal pigmented epithelium, is associated with loss of activity of DICER1, a microRNA-processing enzyme. But cell death is not linked to microRNA processing. Rather, the absence of DICER1 allows pathological Alu repeat sequence RNAs to accumulate. DICER1 binds to and degrades Alu RNA to prevent retinal degeneration. This work suggests a novel therapeutic target for an important cause of blindness. In geographic atrophy, a type of macular degeneration, retinal pigmented epithelium (RPE) cells die. This paper finds that DICER1, which processes miRNA precursors, is reduced in RPE from individuals with geographic atrophy. Cell death is not due to loss of miRNA processing, however; rather, the absence of DICER1 allows accumulation of pathological Alu repeat sequence RNAs. This work reveals a novel function of Dicer in degrading Alu RNAs.