Angewandte Chemie (International ed.), 2010-12, Vol.49 (50), p.9648-9651
2010
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Details
- Autor(en) / Beteiligte
- Titel
- RNA-Directed Packaging of Enzymes within Virus-like Particles
- Ist Teil von
- Angewandte Chemie (International ed.), 2010-12, Vol.49 (50), p.9648-9651
- Ort / Verlag
- Weinheim: Wiley-VCH Verlag
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Packaged molecular machines are available using dual expression vectors that guide the preparation of Qβ virus‐like particles encapsulating multiple enzymes. Packaging is promoted by RNA aptamer sequences that bridge between the coat protein and a peptide tag fused to the desired cargo (see scheme). Peptidase E and luciferase were thus encapsulated and shown to be catalytically active inside the particle.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1433-7851eISSN: 1521-3773DOI: 10.1002/anie.201005243Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3060796
- Format
- –
- Schlagworte
- Allolevivirus - genetics, Allolevivirus - metabolism, Aminopeptidases - administration & dosage, Aminopeptidases - genetics, Aminopeptidases - metabolism, Animals, Capsid Proteins - genetics, Capsid Proteins - metabolism, encapsulation, enzymes, Enzymes, Immobilized - administration & dosage, Enzymes, Immobilized - genetics, Enzymes, Immobilized - metabolism, Escherichia coli - genetics, Fireflies - genetics, Fireflies - metabolism, Gene Expression, Luciferases, Firefly - administration & dosage, Luciferases, Firefly - genetics, Luciferases, Firefly - metabolism, Qβ bacteriophage, RNA, RNA, Messenger - genetics, RNA, Messenger - metabolism, RNA-protein interactions, Virion - genetics, Virion - metabolism, virus-like particles