Details

Autor(en) / Beteiligte

• Nagaike, Takashi
• Logan, Charlotte
• Hotta, Ikuko
• Rozenblatt-Rosen, Orit
• Meyerson, Matthew
• Manley, James L.

Titel

Transcriptional Activators Enhance Polyadenylation of mRNA Precursors

Ist Teil von

• Molecular cell, 2011-02, Vol.41 (4), p.409-418

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2011

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Polyadenylation of mRNA precursors is frequently coupled to transcription by RNA polymerase II. Although this coupling is known to involve interactions with the C-terminal domain of the RNA polymerase II largest subunit, the possible role of other factors is not known. Here we show that a prototypical transcriptional activator, GAL4-VP16, stimulates transcription-coupled polyadenylation in vitro. In the absence of GAL4-VP16, specifically initiated transcripts accumulated but little polyadenylation was observed, while in its presence polyadenylation was strongly enhanced. We further show that this stimulation requires the transcription elongation-associated PAF complex (PAF1c), as PAF1c depletion blocked GAL4-VP16-stimulated polyadenylation. Furthermore, knockdown of PAF subunits by siRNA resulted in decreased 3′ cleavage, and nuclear export, of mRNA in vivo. Finally, we show that GAL4-VP16 interacts directly with PAF1c and recruits it to DNA templates. Our results indicate that a transcription activator can stimulate transcription-coupled 3′ processing and does so via interaction with PAF1c. ► GAL4-VP16 stimulates transcription-coupled polyadenylation ► PAF1c is required for GAL-VP16-stimulated polyadenylation ► GAL4-VP16 interacts directly with PAF1c and recruits it to DNA templates