The journal of clinical endocrinology and metabolism, 2011-03, Vol.96 (3), p.775-781
2011
Details
- Autor(en) / Beteiligte
- Titel
- A High-Fat Diet Elicits Differential Responses in Genes Coordinating Oxidative Metabolism in Skeletal Muscle of Lean and Obese Individuals
- Ist Teil von
- The journal of clinical endocrinology and metabolism, 2011-03, Vol.96 (3), p.775-781
- Ort / Verlag
- Bethesda, MD: Endocrine Society
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- Oxford Journals 2020 Medicine
- Beschreibungen/Notizen
- A 5-day, eucaloric high fat diet up-regulates genes involved in fat metabolism in skeletal muscle of lean, but not obese, individuals. Context: In lean individuals, increasing dietary lipid can elicit an increase in whole body lipid oxidation; however, with obesity the capacity to respond to changes in substrate availability appears to be compromised. Objective: To determine whether the responses of genes regulating lipid oxidation in skeletal muscle differed between lean and insulin resistant obese humans upon exposure to a high-fat diet (HFD). Design and Setting: A 5-d prospective study conducted in the research unit of an academic center. Participants: Healthy, lean (n = 12; body mass index = 22.1 ± 0.6 kg/m2), and obese (n=10; body mass index = 39.6 ± 1.7 kg/m2) males and females, between ages 18 and 30. Intervention: Participants were studied before and after a 5-d HFD (65% fat). Main Outcome Measures: Skeletal muscle biopsies (vastus lateralis) were obtained in the fasted and fed states before and after the HFD and mRNA content for genes involved with lipid oxidation determined. Skeletal muscle acylcarnitine content was determined in the fed states before and after the HFD. Results: Peroxisome proliferator activated receptor (PPAR) α mRNA content increased in lean, but not obese, subjects after a single high-fat meal. From Pre- to Post-HFD, mRNA content exhibited a body size × HFD interaction, where the lean individuals increased while the obese individuals decreased mRNA content for pyruvate dehydrogenase kinase 4, uncoupling protein 3, PPARα, and PPARγ coactivator-1α (P ≤ 0.05). In the obese subjects medium-chain acylcarnitine species tended to accumulate, whereas no change or a reduction was evident in the lean individuals. Conclusions: These findings indicate a differential response to a lipid stimulus in the skeletal muscle of lean and insulin resistant obese humans.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-972XeISSN: 1945-7197DOI: 10.1210/jc.2010-2253Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3047224
- Format
- –
- Schlagworte
- Adolescent, Adult, Age, Biological and medical sciences, Biopsy, Blood Glucose - metabolism, Body Mass Index, Body size, Carnitine - analogs & derivatives, Carnitine - metabolism, dehydrogenase, Diet, Dietary Fats - pharmacology, Endocrine Research, Endocrinopathies, Fatty Acids, Nonesterified - blood, Feeding. Feeding behavior, Female, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation - drug effects, High fat diet, Humans, Insulin, Insulin - blood, Insulin - metabolism, Ion Channels - genetics, Ion Channels - metabolism, Lipid Metabolism - drug effects, Lipid Metabolism - genetics, Lipid peroxidation, Lipids, Male, Medical sciences, Mitochondrial Proteins - genetics, Mitochondrial Proteins - metabolism, mRNA, Muscle, Skeletal - chemistry, Muscle, Skeletal - metabolism, Obesity, Obesity - metabolism, Oxidation-Reduction, Oxidative metabolism, Peroxisome proliferator-activated receptors, PPAR alpha - biosynthesis, PPAR alpha - genetics, Pyruvate Dehydrogenase (Lipoamide) - genetics, Pyruvate Dehydrogenase (Lipoamide) - metabolism, Pyruvic acid, RNA, Messenger - biosynthesis, RNA, Messenger - genetics, Skeletal muscle, Spectrometry, Mass, Electrospray Ionization, Uncoupling Protein 3, Vertebrates: anatomy and physiology, studies on body, several organs or systems, Vertebrates: endocrinology, Young Adult