Vaccine, 2011-01, Vol.29 (3), p.476-486
2011
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- Autor(en) / Beteiligte
- Titel
- A live attenuated Listeria monocytogenes vaccine vector expressing SIV Gag is safe and immunogenic in macaques and can be administered repeatedly
- Ist Teil von
- Vaccine, 2011-01, Vol.29 (3), p.476-486
- Ort / Verlag
- Kidlington: Elsevier Ltd
- Erscheinungsjahr
- 2011
- Quelle
- ScienceDirect
- Beschreibungen/Notizen
- Abstract Listeria monocytogenes (Lm) is known to induce strong cellular immune responses. We constructed a live-attenuated Lm vector, Lmdd-BdopSIVgag , which encodes SIVmac239 gag . Intragastric (i.g.) administration of 3 × 1012 bacteria to rhesus macaques was safe and induced anti-Gag cellular but no humoral immune responses. Boosting of Gag-specific cellular responses was observed after i.g. administration of Lmdd-BdopSIVgag to previously vaccinated RM despite preexisting anti-Lm immunity shown by lymphoproliferative responses. Surprisingly, anti-Lm cellular responses were also detected in non-vaccinated controls, which may reflect the fact that Lm is a ubiquitous bacterium. The novel, live-attenuated Lmdd-BdopSIVgag may be an attractive platform for oral vaccine delivery.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0264-410XeISSN: 1873-2518DOI: 10.1016/j.vaccine.2010.10.072Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3039419
- Format
- –
- Schlagworte
- Acquired immune deficiency syndrome, AIDS, Allergy and Immunology, Animals, Anti-vector immunity, bacteria, Biological and medical sciences, cell-mediated immunity, Cellular immune responses, Drug Carriers, Fundamental and applied biological sciences. Psychology, Gene Products, gag - genetics, Gene Products, gag - immunology, Genetic Vectors, HIV, HIV/AIDS vaccine, Human immunodeficiency virus, humoral immunity, Immune system, Immunity, Cellular, Immunization, Secondary - methods, Infections, Listeria monocytogenes, Listeria monocytogenes - genetics, Live attenuated Listeria monocytogenes, Live oral vaccine, Macaca mulatta, Microbiology, Rodents, SAIDS Vaccines - administration & dosage, SAIDS Vaccines - genetics, SAIDS Vaccines - immunology, Simian immunodeficiency virus, Simian Immunodeficiency Virus - genetics, Simian Immunodeficiency Virus - immunology, Vaccination - methods, Vaccines, Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies, Vaccines, Attenuated - administration & dosage, Vaccines, Attenuated - genetics, Vaccines, Attenuated - immunology, Virology