Clinical and experimental immunology, 2010-12, Vol.162 (3), p.474-486
2010
Details
- Autor(en) / Beteiligte
- Titel
- Inflammatory conditions affect gene expression and function of human adipose tissue‐derived mesenchymal stem cells
- Ist Teil von
- Clinical and experimental immunology, 2010-12, Vol.162 (3), p.474-486
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2010
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- There is emerging interest in the application of mesenchymal stem cells (MSC) for the prevention and treatment of autoimmune diseases, graft‐versus‐host disease and allograft rejection. It is, however, unknown how inflammatory conditions affect phenotype and function of MSC. Adipose tissue‐derived mesenchymal stem cells (ASC) were cultured with alloactivated peripheral blood mononuclear cells (PBMC) (mixed lymphocyte reaction: MLR), with proinflammatory cytokines [interferon (IFN)‐γ, tumour necrosis factor (TNF)‐α and interleukin (IL)‐6] or under control conditions, and their full genome expression and function examined. Proinflammatory cytokines mainly increased indoleamine‐2,3‐dioxygenase expression, whereas ASC cultured with MLR showed increased expression of COX‐2, involved in prostaglandin E₂ production. Both conditions had a stimulatory, but differential, effect on the expression of proinflammatory cytokines and chemokines, while the expression of fibrotic factors was decreased only in response to proinflammatory cytokines. Functional analysis demonstrated that inflammatory conditions affected morphology and proliferation of ASC, while their differentiation capacity and production of trophic factors was unaffected. The immunosuppressive capacity of ASC was enhanced strongly under inflammatory conditions. In conclusion, ASC showed enhanced immunosuppressive capacity under inflammatory conditions, while their differentiation capacity was preserved. Therefore, in vitro preconditioning provides ASC with improved properties for immediate clinical immune therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0009-9104eISSN: 1365-2249DOI: 10.1111/j.1365-2249.2010.04256.xTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3026550
- Format
- –
- Schlagworte
- Adipose Tissue - cytology, Analytical, structural and metabolic biochemistry, Autoimmune diseases, Biological and medical sciences, Cell Proliferation, Cells, Cultured, Chemokines, Coculture Techniques, Cyclooxygenase 2 - biosynthesis, Cyclooxygenase 2 - genetics, Cyclooxygenase-2, Cytokines, Cytokines - pharmacology, Differentiation, Fundamental and applied biological sciences. Psychology, gene array, Gene expression, Gene Expression Profiling, Gene Expression Regulation - immunology, Genomes, Graft rejection, Graft-versus-host reaction, Humans, Immune system, Immunosuppression, Indoleamine-Pyrrole 2,3,-Dioxygenase - genetics, Indoleamine-Pyrrole 2,3,-Dioxygenase - immunology, Indoleamine-Pyrrole 2,3,-Dioxygenase - metabolism, Inflammation, Inflammation Mediators - pharmacology, Interferon, Interleukins, Leukocytes, Mononuclear - immunology, Leukocytes, Mononuclear - metabolism, Lymphocyte Activation, mesenchymal stem cell, Mesenchymal Stromal Cells - cytology, Mesenchymal Stromal Cells - drug effects, Mesenchymal Stromal Cells - immunology, Mesenchymal Stromal Cells - metabolism, Mesenchyme, microarray, Mixed leukocyte reaction, mixed lymphocyte reactions, Peripheral blood mononuclear cells, Prostaglandin E2, Stem cells, Translational Studies, Trophic factors, Tumor necrosis factor