The Journal of biological chemistry, 2010-12, Vol.285 (53), p.41597-41603
2010
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Details
- Autor(en) / Beteiligte
- Titel
- A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain (CTD)
- Ist Teil von
- The Journal of biological chemistry, 2010-12, Vol.285 (53), p.41597-41603
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Spt6 is an essential transcription elongation factor and histone chaperone that binds the C-terminal repeat domain (CTD) of RNA polymerase II. We show here that Spt6 contains a tandem SH2 domain with a novel structure and CTD-binding mode. The tandem SH2 domain binds to a serine 2-phosphorylated CTD peptide in vitro, whereas its N-terminal SH2 subdomain, which we previously characterized, does not. CTD binding requires a positively charged crevice in the C-terminal SH2 subdomain, which lacks the canonical phospho-binding pocket of SH2 domains and had previously escaped detection. The tandem SH2 domain is apparently required for transcription elongation in vivo as its deletion in cells is lethal in the presence of 6-azauracil.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9258eISSN: 1083-351XDOI: 10.1074/jbc.M110.144568Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3009887
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Chaperones, Chromatin, Chromatin - chemistry, Clonal deletion, Crystal Structure, Crystallography, X-Ray - methods, DNA-directed RNA polymerase, Gene Regulation, Histone Chaperones, Histones, Humans, Molecular Sequence Data, Nuclear Proteins - chemistry, Nuclear Proteins - physiology, Peptide Interactions, Peptides - chemistry, Phosphorylation, Protein Structure, Tertiary, RNA Polymerase II, RNA Polymerase II - chemistry, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - chemistry, Saccharomyces cerevisiae Proteins - physiology, Sequence Homology, Amino Acid, Serine, src Homology Domains, Transcription elongation, Transcription Elongation Factors, Transcription, Genetic, Transcriptional Elongation Factors - chemistry, Transcriptional Elongation Factors - physiology