Details

Autor(en) / Beteiligte

• Beattie, Bradley J
• Klose, Alexander D
• Le, Carl H
• Longo, Valerie A
• Dobrenkov, Konstantine
• Vider, Jelena
• Koutcher, Jason A
• Blasberg, Ronald G

Titel

Registration of planar bioluminescence to magnetic resonance and x-ray computed tomography images as a platform for the development of bioluminescence tomography reconstruction algorithms

Ist Teil von

• Journal of Biomedical Optics, 2009-03, Vol.14 (2), p.024045-024049

Ort / Verlag

United States: Society of Photo-Optical Instrumentation Engineers

Erscheinungsjahr

2009

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• The procedures we propose make possible the mapping of two-dimensional (2-D) bioluminescence image (BLI) data onto a skin surface derived from a three-dimensional (3-D) anatomical modality [magnetic resonance (MR) or computed tomography (CT)] dataset. This mapping allows anatomical information to be incorporated into bioluminescence tomography (BLT) reconstruction procedures and, when applied using sources visible to both optical and anatomical modalities, can be used to evaluate the accuracy of those reconstructions. Our procedures, based on immobilization of the animal and determined fixed projective transforms, should be more robust and accurate than previously described efforts, which rely on a poorly constrained retrospectively determined warping of the 3-D anatomical information. Experiments conducted to measure the accuracy of the proposed registration procedure found it to have a mean error of . Additional experiments highlight some of the confounds that are often overlooked in the BLT reconstruction process, and for two of these confounds, simple corrections are proposed.