The Journal of general physiology, 2010-06, Vol.135 (6), p.583-594
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Neuron-astrocyte interactions in the medial nucleus of the trapezoid body
- Ist Teil von
- The Journal of general physiology, 2010-06, Vol.135 (6), p.583-594
- Ort / Verlag
- United States: Rockefeller University Press
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The calyx of Held (CoH) synapse serves as a model system to analyze basic mechanisms of synaptic transmission. Astrocyte processes are part of the synaptic structure and contact both pre- and postsynaptic membranes. In the medial nucleus of the trapezoid body (MNTB), midline stimulation evoked a current response that was not mediated by glutamate receptors or glutamate uptake, despite the fact that astrocytes express functional receptors and transporters. However, astrocytes showed spontaneous Ca(2+) responses and neuronal slow inward currents (nSICs) were recorded in the postsynaptic principal neurons (PPNs) of the MNTB. These currents were correlated with astrocytic Ca(2+) activity because dialysis of astrocytes with BAPTA abolished nSICs. Moreover, the frequency of these currents was increased when Ca(2+) responses in astrocytes were elicited. NMDA antagonists selectively blocked nSICs while D-serine degradation significantly reduced NMDA-mediated currents. In contrast to previous studies in the hippocampus, these NMDA-mediated currents were rarely synchronized.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-1295eISSN: 1540-7748DOI: 10.1085/jgp.200910354Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2888059
- Format
- –
- Schlagworte
- Amino Acid Transport System X-AG - metabolism, Animals, Anthozoa - genetics, Astrocytes - drug effects, Astrocytes - metabolism, Brain Stem - cytology, Brain Stem - drug effects, Brain Stem - metabolism, Calcium, Calcium - metabolism, Cell Communication - drug effects, Chelating Agents - pharmacology, Electric Stimulation, Excitatory Amino Acid Antagonists - pharmacology, Glial Fibrillary Acidic Protein, Glutamic Acid - metabolism, Green Fluorescent Proteins - genetics, Green Fluorescent Proteins - metabolism, Membrane Potentials, Membranes, Mice, Mice, Transgenic, N-Methylaspartate - metabolism, Nerve Tissue Proteins - genetics, Nerve Tissue Proteins - metabolism, Nervous system, Neurons, Neurons - drug effects, Neurons - metabolism, Physiology, Receptors, Glutamate - metabolism, Receptors, N-Methyl-D-Aspartate - metabolism, Recombinant Fusion Proteins - metabolism, Serine - metabolism, Synapses - drug effects, Synapses - metabolism, Synaptic Transmission - drug effects, Time Factors