Details

Autor(en) / Beteiligte

• Kwakkenbos, Mark J.
• Diehl, Sean A.
• Yasuda, Etsuko
• Bakker, Arjen Q.
• van Geelen, Caroline M.M.
• Lukens, Michaël V.
• van Bleek, Grada M.
• Widjojoatmodjo, Myra N.
• Bogers, Willy M.J.M.
• Mei, Henrik
• Radbruch, Andreas
• Scheeren, Ferenc A.
• Spits, Hergen
• Beaumont, Tim

Titel

Generation of stable monoclonal antibody-producing BCR+ human memory B cells by genetic programming

Ist Teil von

• Nature medicine, 2009-12, Vol.16 (1), p.123-128

Erscheinungsjahr

2009

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• B cell lymphoma (BCL)6 and Bcl-xL are expressed in germinal center (GC) B cells and enable them to endure the proliferative and mutagenic environment of the GC. By introducing these genes into peripheral blood memory B cells and culturing these cells with factors produced by follicular helper T cells, CD40L and IL-21, we convert them to highly proliferating, cell surface BCR positive, Ig-secreting B cells with features of GC B cells including expression of activation-induced cytidine deaminase. We generated cloned lines of B cells specific for respiratory syncytial virus and used these cells as a source of antibodies that effectively neutralized this virus in vivo . This method provides a new tool to study GC B cell biology, signal transduction through antigen-specific B cell receptors, and for the rapid generation of high affinity human monoclonal antibodies.