Details

Autor(en) / Beteiligte

• Eser, Bekir E
• Barr, Eric W
• Frantom, Patrick A
• Saleh, Lana
• Bollinger, J. Martin
• Krebs, Carsten
• Fitzpatrick, Paul F

Titel

Direct Spectroscopic Evidence for a High-Spin Fe(IV) Intermediate in Tyrosine Hydroxylase

Ist Teil von

• Journal of the American Chemical Society, 2007-09, Vol.129 (37), p.11334-11335

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2007

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Tyrosine hydroxylase, a member of the aromatic amino acid hydroxylase family, uses a mononuclear Fe(II) and tetrahydropterin for hydroxylation of tyrosine to dihydroxyphenylalanine. Rapid-freeze quench Mössbauer spectroscopy has now provided direct evidence for the presence of an Fe(IV) intermediate in the reaction catalyzed by tyrosine hydroxylase. Rapid-quench techniques provide support for the kinetic competence of this species as the hydroxylating intermediate. This is the first direct evidence for a mononuclear Fe(IV) intermediate in an enzymatic aromatic hydroxylation reaction.