Science (American Association for the Advancement of Science), 2007-03, Vol.315 (5816), p.1270-1274
2007
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Reconstitution of DNA Segregation Driven by Assembly of a Prokaryotic Actin Homolog
- Ist Teil von
- Science (American Association for the Advancement of Science), 2007-03, Vol.315 (5816), p.1270-1274
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2007
- Quelle
- Science Online_科学在线
- Beschreibungen/Notizen
- Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1138527Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2851738
- Format
- –
- Schlagworte
- Actins, Actins - chemistry, Actins - metabolism, Actins - ultrastructure, Adenosine Triphosphate - metabolism, Bacterial Proteins - metabolism, Bacteriology, Biochemistry, Biological and medical sciences, Biopolymers, Cell cycle, cell proliferation, Cell physiology, Cellular biology, Deoxyribonucleic acid, DNA, DNA Topoisomerase IV - metabolism, DNA, Bacterial - metabolism, Escherichia coli Proteins - chemistry, Escherichia coli Proteins - metabolism, Escherichia coli Proteins - ultrastructure, Fluorescence, Fundamental and applied biological sciences. Psychology, Genetics, Growth, nutrition, cell differenciation, Irradiation, Lasers, Microbiology, Microscopy, Microspheres, Molecular and cellular biology, Monomers, Plasmids, Polymerization, Protein Binding, Proteins, R Factors - genetics, R Factors - metabolism, Repressor Proteins - metabolism, Steady states