Science (American Association for the Advancement of Science), 2009-05, Vol.324 (5929), p.938-941
2009
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- Autor(en) / Beteiligte
- Titel
- MAPK3/1 (ERK1/2) in Ovarian Granulosa Cells Are Essential for Female Fertility
- Ist Teil von
- Science (American Association for the Advancement of Science), 2009-05, Vol.324 (5929), p.938-941
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2009
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- A surge of luteinizing hormone (LH) from the pituitary gland triggers ovulation, oocyte maturation, and luteinization for successful reproduction in mammals. Because the signaling molecules RAS and ERK1/2 (extracellular signal-regulated kinases 1 and 2) are activated by an LH surge in granulosa cells of preovulatory follicles, we disrupted Erk1/2 in mouse granulosa cells and provide in vivo evidence that these kinases are necessary for LH-induced oocyte resumption of meiosis, ovulation, and luteinization. In addition, biochemical analyses and selected disruption of the Cebpb gene in granulosa cells demonstrate that C/EBPβ (CCAAT/Enhancer-binding protein-β) is a critical downstream mediator of ERK1/2 activation. Thus, ERK1/2 and C/EBPβ constitute an in vivo LH-regulated signaling pathway that controls ovulation- and luteinization-related events.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1171396Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2847890
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, CCAAT-Enhancer-Binding Protein-beta - genetics, CCAAT-Enhancer-Binding Protein-beta - metabolism, Cellular biology, Endocrinology, Enzyme Activation, Female, Females, Fertility, Follicles, Fundamental and applied biological sciences. Psychology, Gene Expression Profiling, Gene expression regulation, Granulosa cells, Granulosa Cells - enzymology, Granulosa Cells - metabolism, Hormones, Luteal cells, Luteinization, Luteinizing Hormone - metabolism, MAP Kinase Signaling System, Mating behavior, Meiosis, Mice, Mice, Inbred C57BL, Mice, Transgenic, Mitogen-Activated Protein Kinase 1 - metabolism, Mitogen-Activated Protein Kinase 3 - metabolism, Oocytes, Oocytes - physiology, Ovarian Follicle - physiology, Ovaries, Ovulation, Phosphorylation, Reproductive system, Rodents, Signal transduction, Vertebrates: nervous system and sense organs