Acta crystallographica. Section F, Structural biology and crystallization communications, 2009-11, Vol.65 (11), p.1193-1196
2009
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Details
- Autor(en) / Beteiligte
- Titel
- Expression, purification and crystallization of an archaeal-type phosphoenolpyruvate carboxylase
- Ist Teil von
- Acta crystallographica. Section F, Structural biology and crystallization communications, 2009-11, Vol.65 (11), p.1193-1196
- Ort / Verlag
- 5 Abbey Square, Chester, Cheshire CH1 2HU, England: International Union of Crystallography
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- An archaeal‐type phosphoenolpyruvate carboxylase (PepcA) from Clostridium perfringens has been expressed in Escherichia coli in a soluble form with an amino‐terminal His tag. The recombinant protein is enzymatically active and two crystal forms have been obtained. Complete diffraction data extending to 3.13 Å resolution have been measured from a crystal soaked in KAu(CN)2, using radiation at a wavelength just above the Au LIII edge. The asymmetric unit contains two tetramers of PepcA.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1744-3091eISSN: 1744-3091DOI: 10.1107/S1744309109042663Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2777057
- Format
- –
- Schlagworte
- Archaeal Proteins - chemistry, Archaeal Proteins - genetics, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Clostridium perfringens, Clostridium perfringens - enzymology, Crystallization, Crystallization Communications, Crystallography, X-Ray, Isoenzymes - chemistry, Isoenzymes - genetics, Molecular Sequence Data, phosphoenolpyruvate carboxylase, Phosphoenolpyruvate Carboxylase - chemistry, Phosphoenolpyruvate Carboxylase - genetics, Recombinant Proteins - chemistry, Recombinant Proteins - genetics, X-Ray Diffraction