Nutrition and cancer, 2009-01, Vol.61 (4), p.554-563
2009
Details
- Autor(en) / Beteiligte
- Titel
- Kaempferol Inhibits Angiogenesis and VEGF Expression Through Both HIF Dependent and Independent Pathways in Human Ovarian Cancer Cells
- Ist Teil von
- Nutrition and cancer, 2009-01, Vol.61 (4), p.554-563
- Ort / Verlag
- Philadelphia, PA: Taylor & Francis Group
- Erscheinungsjahr
- 2009
- Link zum Volltext
- Quelle
- Taylor & Francis Journals Auto-Holdings Collection
- Beschreibungen/Notizen
- Ovarian cancer is 1 of the most significant malignancies in the Western world, and the antiangiogenesis strategy has been postulated for prevention and treatment of ovarian cancers. Kaempferol is a natural flavonoid present in many fruits and vegetables. The antiangiogenesis potential of kaempferol and its underlying mechanisms were investigated in two ovarian cancer cell lines, OVCAR-3 and A2780/CP70. Kaempferol mildly inhibits cell viability but significantly reduces VEGF gene expression at mRNA and protein levels in both ovarian cancer cell lines. In chorioallantoic membranes of chicken embryos, kaempferol significantly inhibits OVCAR-3-induced angiogenesis and tumor growth. HIF-1, a regulator of VEGF, is downregulated by kaempferol treatment in both ovarian cancer cell lines. Kaempferol also represses AKT phosphorylation dose dependently at 5 to 20 μM concentrations. ESRRA is a HIF-independent VEGF regulator, and it is also downregulated by kaempferol in a dose-dependent manner. Overall, this study demonstrated that kaempferol is low in cytotoxicity but inhibits angiogenesis and VEGF expression in human ovarian cancer cells through both HIF-dependent (Akt/HIF) and HIF-independent (ESRRA) pathways and deserves further studies for possible application in angio prevention and treatment of ovarian cancers.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0163-5581eISSN: 1532-7914DOI: 10.1080/01635580802666281Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2770884
- Format
- –
- Schlagworte
- Analysis of Variance, angiogenesis growth factor, Angiogenesis Inhibitors - administration & dosage, Angiogenesis Inhibitors - pharmacology, Animals, anticarcinogenic activity, biochemical mechanisms, Biological and medical sciences, carcinogenesis, cell culture, Cell Line, Tumor, Cell Proliferation - drug effects, Cell Survival - drug effects, chemical constituents of plants, Chick Embryo, Chorioallantoic Membrane - blood supply, Dose-Response Relationship, Drug, ERRalpha Estrogen-Related Receptor, Extracellular Signal-Regulated MAP Kinases - metabolism, Feeding. Feeding behavior, Female, Female genital diseases, fruits (food), Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Neoplastic - drug effects, Gynecology. Andrology. Obstetrics, Heat-Shock Proteins - genetics, Heat-Shock Proteins - metabolism, Humans, Hypoxia-Inducible Factor 1 - genetics, Hypoxia-Inducible Factor 1 - metabolism, kaempferol, Kaempferols - administration & dosage, Kaempferols - genetics, Kaempferols - metabolism, Kaempferols - pharmacology, mechanism of action, Medical sciences, nutrition-genotype interaction, nutritional intervention, ovarian neoplasms, Ovarian Neoplasms - genetics, Ovarian Neoplasms - metabolism, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Phosphorylation - drug effects, protein synthesis, Proto-Oncogene Proteins c-akt - metabolism, Receptors, Estrogen - genetics, Receptors, Estrogen - metabolism, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors - genetics, Transcription Factors - metabolism, Tumor Suppressor Protein p53 - deficiency, Tumor Suppressor Protein p53 - genetics, Tumors, Vascular Endothelial Growth Factors - genetics, Vascular Endothelial Growth Factors - metabolism, vegetables, Vertebrates: anatomy and physiology, studies on body, several organs or systems, women, Xenograft Model Antitumor Assays