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  • BibTeX
Drosophila hairpin RNA pathway generates endogenous short interfering RNAs
Nature, 2008-06, Vol.453 (7196), p.803-806
2008

Details

Autor(en) / Beteiligte
Titel
Drosophila hairpin RNA pathway generates endogenous short interfering RNAs
Ist Teil von
  • Nature, 2008-06, Vol.453 (7196), p.803-806
Ort / Verlag
London: Nature Publishing
Erscheinungsjahr
2008
Link zum Volltext
Quelle
EBSCOhost Psychology and Behavioral Sciences Collection
Beschreibungen/Notizen
  • In contrast to microRNAs and Piwi-associated RNAs, short interfering RNAs (siRNAs) are seemingly dispensable for host-directed gene regulation in Drosophila. This notion is based on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology. Moreover, endogenous Drosophila siRNAs have not yet been identified. Here we report that siRNAs derived from long hairpin RNA genes (hpRNAs) programme Slicer complexes that can repress endogenous target transcripts. The Drosophila hpRNA pathway is a hybrid mechanism that combines canonical RNA interference factors (Dicer-2, Hen1 (known as CG12367) and Argonaute 2) with a canonical microRNA factor (Loquacious) to generate 21-nucleotide siRNAs. These novel regulatory RNAs reveal unexpected complexity in the sorting of small RNAs, and open a window onto the biological usage of endogenous RNA interference in Drosophila.
Sprache
Englisch
Identifikatoren
ISSN: 0028-0836
eISSN: 1476-4687, 1476-4679
DOI: 10.1038/nature07015
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2735555
Format
–
Schlagworte
Animals, Argonaute Proteins, binding capacity, Biological and medical sciences, Cells, Drosophila, Drosophila melanogaster, Drosophila melanogaster - enzymology, Drosophila melanogaster - genetics, Drosophila melanogaster - metabolism, Drosophila Proteins - genetics, Drosophila Proteins - metabolism, Fundamental and applied biological sciences. Psychology, gene expression, genes, hairpin RNA, Insects, Methyltransferases - metabolism, MicroRNAs - biosynthesis, MicroRNAs - genetics, MicroRNAs - metabolism, Molecular and cellular biology, Molecular genetics, Nucleic Acid Conformation, Proteins, regulatory RNA, Ribonuclease III, RNA, RNA Helicases - genetics, RNA Helicases - metabolism, RNA Interference, RNA, Double-Stranded - chemistry, RNA, Double-Stranded - genetics, RNA, Double-Stranded - metabolism, RNA, Small Interfering - biosynthesis, RNA, Small Interfering - genetics, RNA, Small Interfering - metabolism, RNA-binding proteins, RNA-Binding Proteins - genetics, RNA-Binding Proteins - metabolism, RNA-Induced Silencing Complex - genetics, RNA-Induced Silencing Complex - metabolism, small interfering RNA, Transcription. Transcription factor. Splicing. Rna processing, Transfer RNA

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