Blood, 2009-07, Vol.114 (2), p.346-356
2009
Details
- Autor(en) / Beteiligte
- Titel
- IL-10 is up-regulated in multiple cell types during viremic HIV infection and reversibly inhibits virus-specific T cells
- Ist Teil von
- Blood, 2009-07, Vol.114 (2), p.346-356
- Ort / Verlag
- Washington, DC: Elsevier Inc
- Erscheinungsjahr
- 2009
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Murine models indicate that interleukin-10 (IL-10) can suppress viral clearance, and interventional blockade of IL-10 activity has been proposed to enhance immunity in chronic viral infections. Increased IL-10 levels have been observed during HIV infection and IL-10 blockade has been shown to enhance T-cell function in some HIV-infected subjects. However, the categories of individuals in whom the IL-10 pathway is up-regulated are poorly defined, and the cellular sources of IL-10 in these subjects remain to be determined. Here we report that blockade of the IL-10 pathway augmented in vitro proliferative capacity of HIV-specific CD4 and CD8 T cells in individuals with ongoing viral replication. IL-10 blockade also increased cytokine secretion by HIV-specific CD4 T cells. Spontaneous IL-10 expression, measured as either plasma IL-10 protein or IL-10 mRNA in peripheral blood mononuclear cells (PBMCs), correlated positively with viral load and diminished after successful antiretroviral therapy. IL-10 mRNA levels were up-regulated in multiple PBMC subsets in HIV-infected subjects compared with HIV-negative controls, particularly in T, B, and natural killer (NK) cells, whereas monocytes were a major source of IL-10 mRNA in HIV-infected and -uninfected individuals. These data indicate that multiple cell types contribute to IL-10–mediated immune suppression in the presence of uncontrolled HIV viremia.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-4971eISSN: 1528-0020DOI: 10.1182/blood-2008-12-191296Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2714209
- Format
- –
- Schlagworte
- Biological and medical sciences, Cell Proliferation, Cells, Cultured, Disease, Gene Expression Regulation, Hematologic and hematopoietic diseases, HIV Infections - complications, HIV Infections - genetics, HIV Infections - immunology, HIV Infections - metabolism, Human viral diseases, Humans, Immunobiology, Infectious diseases, Interleukin-10 - genetics, Interleukin-10 - immunology, Interleukin-10 - metabolism, Medical sciences, Receptors, Interleukin-10 - immunology, RNA, Messenger - genetics, Signal Transduction - immunology, T-Lymphocytes - cytology, T-Lymphocytes - immunology, T-Lymphocytes - metabolism, Up-Regulation - immunology, Viral diseases, Viral diseases of the lymphoid tissue and the blood. Aids, Viremia - complications, Viremia - genetics, Viremia - immunology, Viremia - metabolism