Molecular cell, 2009-04, Vol.34 (1), p.47-57
2009
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- Autor(en) / Beteiligte
- Titel
- A Conserved WD40 Protein Binds the Cajal Body Localization Signal of scaRNP Particles
- Ist Teil von
- Molecular cell, 2009-04, Vol.34 (1), p.47-57
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Small Cajal body (CB)-specific RNPs (scaRNPs) function in posttranscriptional modification of small nuclear (sn)RNAs. An RNA element, the CAB box, facilitates CB localization of H/ACA scaRNPs. Using a related element in Drosophila C/D scaRNAs, we purified a fly WD40 repeat protein that UV crosslinks to RNA in a C/D CAB box-dependent manner and associates with C/D and mixed domain C/D-H/ACA scaRNAs. Its human homolog, WDR79, associates with C/D, H/ACA, and mixed domain scaRNAs, as well as with telomerase RNA. WDR79's binding to human H/ACA and mixed domain scaRNAs is CAB box dependent, and its association with mixed domain RNAs also requires the ACA motif, arguing for additional interactions of WDR79 with H/ACA core proteins. We demonstrate a requirement for WDR79 binding in the CB localization of a scaRNA. This and other recent reports establish WDR79 as a central player in the localization and processing of nuclear RNPs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/j.molcel.2009.02.020Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2700737
- Format
- –
- Schlagworte
- Amino Acid Motifs, Animals, Base Sequence, Cell Line, Chromatography, Affinity, Coiled Bodies - metabolism, Coiled Bodies - ultrastructure, Drosophila melanogaster - genetics, Drosophila melanogaster - metabolism, Drosophila Proteins - analysis, Drosophila Proteins - chemistry, Drosophila Proteins - metabolism, HeLa Cells, Humans, Molecular Sequence Data, Nucleic Acid Conformation, PROTEINS, Recombinant Fusion Proteins - analysis, Regulatory Sequences, Ribonucleic Acid, Ribonucleoproteins - analysis, Ribonucleoproteins - metabolism, RNA, RNA-Binding Proteins - analysis, RNA-Binding Proteins - chemistry, RNA-Binding Proteins - metabolism, Sequence Alignment