Details

Autor(en) / Beteiligte

• Miranda, Fabiana A
• Hassumi, Marcela K
• Guimaraes, Marcia C.M
• Simoes, Renata T
• Silva, Tarsia G.A
• Lira, Regia C.P
• Rocha, Ana M
• Mendes, Celso T., Jr
• Donadi, Eduardo A
• Soares, Christiane P
• Soares, Edson G

Titel

Galectin-3 Overexpression in Invasive Laryngeal Carcinoma, Assessed by Computer-assisted Analysis

Ist Teil von

• The journal of histochemistry and cytochemistry, 2009-07, Vol.57 (7), p.665-673

Ort / Verlag

Los Angeles, CA: Histochemical Soc

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• The larynx is the most common site of malignancy in the upper aerodigestive tract. In Brazil, malignant laryngeal lesions represent 2% of all cancers, with ∼3000 annual deaths. The association between human papillomavirus (HPV) and laryngeal cancer is still controversial. The aim of the present retrospective study was to determine the expression of galectin-3 immunoperoxidase in laryngeal carcinoma by examining paraffin-embedded larynx biopsies from 65 patients, 10 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastases, and 28 with metastases. Twenty-eight cervical lymph nodes from patients with metastatic lesions were also evaluated. Nested PCR was performed to detect and type HPV DNA. Galectin-3 expression was assessed by immunohistochemistry using a computer-assisted system. Among 65 patients, 55 (84.6%) were positive to beta-globin (internal control); 10 (15.4%) patients were beta-globin negative and were excluded from the HPV evaluation. Thus, 7 (12.7%) out of 55 patients were HPV positive and 48 (87.3%) out of 55 patients were HPV negative. High expression of galectin-3 was observed in invasive laryngeal tumors, suggesting that galectin-3 could be associated with the invasiveness and aggressiveness of laryngeal carcinomas. (J Histochem Cytochem 57:665–673, 2009)