Details

Autor(en) / Beteiligte

• Zhang, Qi
• Li, Yulong
• Tsien, Richard W

Titel

Dynamic Control of Kiss-And-Run and Vesicular Reuse Probed with Single Nanoparticles

Ist Teil von

• Science (American Association for the Advancement of Science), 2009-03, Vol.323 (5920), p.1448-1453

Ort / Verlag

Washington, DC: American Association for the Advancement of Science

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single quantum dots. Their size and pH-dependent photoluminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycling. Kiss-and-run dominated at the beginning of stimulus trains, reflecting the preference of vesicles with high release probability. Its incidence was increased by rapid firing, a response appropriate to shape the kinetics of neurotransmission during a wide range of firing patterns.