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Expression profiling identifies microRNA signature in pancreatic cancer
International journal of cancer, 2007-03, Vol.120 (5), p.1046-1054
Lee, Eun Joo
Gusev, Yuriy
Jiang, Jinmai
Nuovo, Gerard J.
Lerner, Megan R.
Frankel, Wendy L.
Morgan, Daniel L.
Postier, Russell G.
Brackett, Daniel J.
Schmittgen, Thomas D.
2007
Details
Autor(en) / Beteiligte
Lee, Eun Joo
Gusev, Yuriy
Jiang, Jinmai
Nuovo, Gerard J.
Lerner, Megan R.
Frankel, Wendy L.
Morgan, Daniel L.
Postier, Russell G.
Brackett, Daniel J.
Schmittgen, Thomas D.
Titel
Expression profiling identifies microRNA signature in pancreatic cancer
Ist Teil von
International journal of cancer, 2007-03, Vol.120 (5), p.1046-1054
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2007
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
microRNAs are functional, 22 nt, noncoding RNAs that negatively regulate gene expression. Disturbance of microRNA expression may play a role in the initiation and progression of certain diseases. A microRNA expression signature has been identified that is associated with pancreatic cancer. This has been accomplished with the application of real‐time PCR profiling of over 200 microRNA precursors on specimens of human pancreatic adenocarcinoma, paired benign tissue, normal pancreas, chronic pancreatitis and nine pancreatic cancer cell lines. Hierarchical clustering was able to distinguish tumor from normal pancreas, pancreatitis and cell lines. The PAM algorithm correctly classified 28 of 28 tumors, 6 of 6 normal pancreas and 11 of 15 adjacent benign tissues. One hundred microRNA precursors were aberrantly expressed in pancreatic cancer or desmoplasia (p < 0.01), including microRNAs previously reported as differentially expressed in other human cancers (miR‐155, miR‐21, miR‐221 and miR‐222) as well as those not previously reported in cancer (miR‐376a and miR‐301). Most of the top aberrantly expressed miRNAs displayed increased expression in the tumor. Expression of the active, mature microRNA was validated using a real‐time PCR assay to quantify the mature microRNA and Northern blotting. Reverse transcription in situ PCR showed that three of the top differentially expressed miRNAs (miR‐221, ‐376a and ‐301) were localized to tumor cells and not to stroma or normal acini or ducts. Aberrant microRNA expression may offer new clues to pancreatic tumorigenesis and may provide diagnostic biomarkers for pancreatic adenocarcinoma. © 2006 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0020-7136
eISSN: 1097-0215
DOI: 10.1002/ijc.22394
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2680248
Format
–
Schlagworte
Adenocarcinoma - chemistry
,
Adenocarcinoma - genetics
,
Adenocarcinoma - pathology
,
Biological and medical sciences
,
cancer
,
Gastroenterology. Liver. Pancreas. Abdomen
,
gene expression
,
Gene Expression Profiling
,
Humans
,
Liver. Biliary tract. Portal circulation. Exocrine pancreas
,
Medical sciences
,
MicroRNAs - analysis
,
MicroRNAs - genetics
,
noncoding RNA
,
Oligonucleotide Array Sequence Analysis
,
Pancreas - chemistry
,
Pancreas - metabolism
,
Pancreas - pathology
,
Pancreatic Neoplasms - chemistry
,
Pancreatic Neoplasms - genetics
,
Pancreatic Neoplasms - pathology
,
real‐time PCR
,
Tumor Cells, Cultured
,
Tumors
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