Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
FoxO1 Controls Insulin-dependent Adipose Triglyceride Lipase (ATGL) Expression and Lipolysis in AdipocytesS
Ist Teil von
The Journal of biological chemistry, 2009-05, Vol.284 (20), p.13296-13300
Ort / Verlag
American Society for Biochemistry and Molecular Biology
Erscheinungsjahr
2009
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
FoxO1 represents a central regulator of metabolism in several cell types.
Although FoxO1 is abundant in adipocytes, its biological functions in these
cells remain largely unknown. We show here that the promotor region of the
rate-limiting lipolytic enzyme, adipose triglyceride lipase (ATGL), has two
FoxO1-binding sites, and co-transfection with wild type and unphosphorylated
FoxO1 mutant activates the expression of luciferase driven by the ATGL
promotor. In 3T3-L1 adipocytes, insulin controls nucleo-cytoplasmic shuttling
of FoxO1 and regulates its interaction with endogenous ATGL promotors.
Knockdown of FoxO1 in 3T3-L1 adipocytes decreases the expression of ATGL and
attenuates basal and isoproterenol-stimulated lipolysis. Infection of mouse
embryonic fibroblasts with FoxO1-encoding lentivirus increases ATGL expression
and renders it sensitive to regulation by insulin. Thus, FoxO1 may play an
important role in the regulation of lipolysis in adipocytes by controlling the
expression of ATGL.