The Plant cell, 2009-02, Vol.21 (2), p.568-580
2009
Details
- Autor(en) / Beteiligte
- Titel
- E3 Ubiquitin Ligase SCFTIR¹/AFB and Membrane Sterols Play Key Roles in Auxin Regulation of Endocytosis, Recycling, and Plasma Membrane Accumulation of the Auxin Efflux Transporter PIN2 in Arabidopsis thaliana
- Ist Teil von
- The Plant cell, 2009-02, Vol.21 (2), p.568-580
- Ort / Verlag
- United States: American Society of Plant Biologists
- Erscheinungsjahr
- 2009
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The PIN family of auxin efflux transporters exhibit polar plasma membrane (PM) localization and play a key role in auxin gradient-mediated developmental processes. Auxin inhibits PIN2 endocytosis and promotes its PM localization. However, the underlying mechanisms remain elusive. Here, we show that the inhibitory effect of auxin on PIN2 endocytosis was impaired in SCFTIR¹/AFB auxin signaling mutants. Similarly, reducing membrane sterols impaired auxin inhibition of PIN2 endocytosis. Gas chromatography-mass spectrometry analyses indicate that membrane sterols were significantly reduced in SCFTIR¹/AFB mutants, supporting a link between membrane sterols and auxin signaling in regulating PIN2 endocytosis. We show that auxin promoted PIN2 recycling from endosomes to the PM and increased PIN2 steady state levels in the PM fraction. Furthermore, we show that the positive effect of auxin on PIN2 levels in the PM was impaired by inhibiting membrane sterols or auxin signaling. Consistent with this, the sterol biosynthetic mutant fk-J79 exhibited pronounced defects in primary root elongation and gravitropic response. Our data collectively indicate that, although there are distinct processes involved in endocytic regulation of specific PM-resident proteins, the SCFTIR¹/AFB-dependent processes are required for auxin regulation of endocytosis, recycling, and PM accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1040-4651eISSN: 1532-298XDOI: 10.1105/tpc.108.061465Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2660622
- Format
- –
- Schlagworte
- Arabidopsis - enzymology, Arabidopsis - growth & development, Arabidopsis - metabolism, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Arabidopsis Proteins - physiology, Cell Membrane - chemistry, Cell Membrane - metabolism, Endocytosis - genetics, Endocytosis - physiology, F-Box Proteins - genetics, F-Box Proteins - metabolism, F-Box Proteins - physiology, Gas Chromatography-Mass Spectrometry, Glycoproteins - genetics, Glycoproteins - metabolism, Glycoproteins - physiology, Gravitropism - physiology, Green Fluorescent Proteins - analysis, Indoleacetic Acids - metabolism, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Proteins - physiology, Plant Roots - enzymology, Plant Roots - growth & development, Plant Roots - metabolism, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, Receptors, Cell Surface - physiology, Recombinant Fusion Proteins - analysis, Signal Transduction - genetics, SKP Cullin F-Box Protein Ligases - genetics, SKP Cullin F-Box Protein Ligases - metabolism, SKP Cullin F-Box Protein Ligases - physiology, Sterols - chemistry, Sterols - metabolism