EMBO reports, 2009-02, Vol.10 (2), p.173-179
2009
Details
- Autor(en) / Beteiligte
- Titel
- PLIC proteins or ubiquilins regulate autophagy-dependent cell survival during nutrient starvation
- Ist Teil von
- EMBO reports, 2009-02, Vol.10 (2), p.173-179
- Ort / Verlag
- Chichester, UK: John Wiley & Sons, Ltd
- Erscheinungsjahr
- 2009
- Link zum Volltext
- Quelle
- Wiley Blackwell Single Titles
- Beschreibungen/Notizen
- Ubiquilins (UBQLNs) are adaptor proteins thought to deliver ubiquitinated substrates to proteasomes. Here, we show a role for UBQLN in autophagy: enforced expression of UBQLN protects cells from starvation‐induced death, whereas depletion of UBQLN renders cells more susceptible. The UBQLN protective effect requires the autophagy‐related genes ATG5 and ATG7, two essential components of autophagy. The ubiquitin‐associated domain of UBQLN mediates both its association with autophagosomes and its protective effect against starvation. Depletion of UBQLN delays the delivery of autophagosomes to lysosomes. This study identifies a new role for UBQLN in regulating the maturation of autophagy, expanding the involvement of ubiquitin‐related proteins in this process.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1469-221XeISSN: 1469-3178DOI: 10.1038/embor.2008.238Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2637314
- Format
- –
- Schlagworte
- Adaptor Proteins, Signal Transducing, Adaptor Proteins, Vesicular Transport - antagonists & inhibitors, Adaptor Proteins, Vesicular Transport - genetics, Adaptor Proteins, Vesicular Transport - physiology, Animals, autophagosomes, autophagy, Autophagy - drug effects, Autophagy - physiology, Autophagy-Related Protein 5, Autophagy-Related Protein 7, Autophagy-Related Proteins, Carrier Proteins - antagonists & inhibitors, Carrier Proteins - genetics, Carrier Proteins - physiology, Cell Cycle Proteins - antagonists & inhibitors, Cell Cycle Proteins - genetics, Cell Cycle Proteins - physiology, Culture Media - pharmacology, Genetics, HeLa Cells - cytology, HeLa Cells - drug effects, Humans, Mice, Microscopy, Immunoelectron, Microtubule-Associated Proteins - antagonists & inhibitors, Microtubule-Associated Proteins - genetics, Microtubule-Associated Proteins - metabolism, Microtubule-Associated Proteins - physiology, Molecular biology, Nutrients, Phagosomes - metabolism, PLIC, Protein Interaction Mapping, Protein Structure, Tertiary, Proteins, Recombinant Fusion Proteins - antagonists & inhibitors, Recombinant Fusion Proteins - physiology, RNA Interference, RNA, Small Interfering - physiology, Scientific Report, Substrates, ubiquilin, Ubiquitin-Activating Enzymes - antagonists & inhibitors, Ubiquitin-Activating Enzymes - genetics, Ubiquitin-Activating Enzymes - physiology, ubiquitin-like, Ubiquitins - antagonists & inhibitors, Ubiquitins - genetics, Ubiquitins - physiology