Details

Autor(en) / Beteiligte

• Koschubs, Tobias
• Seizl, Martin
• Larivière, Laurent
• Kurth, Fabian
• Baumli, Sonja
• Martin, Dietmar E
• Cramer, Patrick

Titel

Identification, structure, and functional requirement of the Mediator submodule Med7N/31

Ist Teil von

• The EMBO journal, 2009-01, Vol.28 (1), p.69-80

Ort / Verlag

Chichester, UK: John Wiley & Sons, Ltd

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Mediator is a modular multiprotein complex required for regulated transcription by RNA polymerase (Pol) II. Here, we show that the middle module of the Mediator core contains a submodule of unique structure and function that comprises the N‐terminal part of subunit Med7 (Med7N) and the highly conserved subunit Med31 (Soh1). The Med7N/31 submodule shows a conserved novel fold, with two proline‐rich stretches in Med7N wrapping around the right‐handed four‐helix bundle of Med31. In vitro , Med7N/31 is required for activated transcription and can act in trans when added exogenously. In vivo , Med7N/31 has a predominantly positive function on the expression of a specific subset of genes, including genes involved in methionine metabolism and iron transport. Comparative phenotyping and transcriptome profiling identify specific and overlapping functions of different Mediator submodules.