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Proteins interacting with Caenorhabditis elegans Gα subunits
Comparative and functional genomics, 2003-10, Vol.4 (5), p.479-491
Cuppen, Edwin
van der Linden, Alexander M.
Jansen, Gert
Plasterk, Ronald H. A.
2003
Details
Autor(en) / Beteiligte
Cuppen, Edwin
van der Linden, Alexander M.
Jansen, Gert
Plasterk, Ronald H. A.
Titel
Proteins interacting with Caenorhabditis elegans Gα subunits
Ist Teil von
Comparative and functional genomics, 2003-10, Vol.4 (5), p.479-491
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2003
Link zum Volltext
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
To identify novel components in heterotrimeric G‐protein signalling, we performed an extensive screen for proteins interacting with Caenorhabditis elegans Gα subunits. The genome of C. elegans contains homologues of each of the four mammalian classes of Gα subunits (Gs, Gi/o, Gq and G12), and 17 other Gα subunits. We tested 19 of the Gα subunits and four constitutively activated Gα subunits in a large‐scale yeast two‐hybrid experiment. This resulted in the identification of 24 clones, representing 11 different proteins that interact with four different Gα subunits. This set includes C. elegans orthologues of known interactors of Gα subunits, such as AGS3 (LGN/PINS), CalNuc and Rap1Gap, but also novel proteins, including two members of the nuclear receptor super family and a homologue of human haspin (germ cell‐specific kinase). All interactions were found to be unique for a specific Gα subunit but variable for the activation status of the Gα subunit. We used expression pattern and RNA interference analysis of the G‐protein interactors in an attempt to substantiate the biological relevance of the observed interactions. Furthermore, by means of a membrane recruitment assay, we found evidence that GPA‐7 and the nuclear receptor NHR‐22 can interact in the animal. Copyright © 2003 John Wiley & Sons, Ltd.
Sprache
Englisch
Identifikatoren
ISSN: 1531-6912
eISSN: 1532-6268
DOI: 10.1002/cfg.318
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2447299
Format
–
Schlagworte
Biological and medical sciences
,
Caenorhabditis elegans
,
Fundamental and applied biological sciences. Psychology
,
General aspects
,
heterotrimeric G-protein
,
Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects)
,
nuclear receptor
,
protein interaction
,
signal transduction
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