Details
- Autor(en) / Beteiligte
- Titel
- Dual Fatty Acyl Modification Determines the Localization and Plasma Membrane Targeting of CBL/CIPK Ca²⁺ Signaling Complexes in Arabidopsis
- Ist Teil von
- The Plant cell, 2008-05, Vol.20 (5), p.1346-1362
- Ort / Verlag
- United States: American Society of Plant Biologists
- Erscheinungsjahr
- 2008
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Arabidopsis thaliana calcineurin B-like proteins (CBLs) interact specifically with a group of CBL-interacting protein kinases (CIPKs). CBL/CIPK complexes phosphorylate target proteins at the plasma membrane. Here, we report that dual lipid modification is required for CBL1 function and for localization of this calcium sensor at the plasma membrane. First, myristoylation targets CBL1 to the endoplasmic reticulum. Second, S-acylation is crucial for endoplasmic reticulum-to-plasma membrane trafficking via a novel cellular targeting pathway that is insensitive to brefeldin A. We found that a 12-amino acid peptide of CBL1 is sufficient to mediate dual lipid modification and to confer plasma membrane targeting. Moreover, the lipid modification status of the calcium sensor moiety determines the cellular localization of preassembled CBL/CIPK complexes. Our findings demonstrate the importance of S-acylation for regulating the spatial accuracy of Ca²⁺-decoding proteins and suggest a novel mechanism that enables the functional specificity of calcium sensor/kinase complexes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1040-4651, 1532-298XeISSN: 1532-298XDOI: 10.1105/tpc.108.058123Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2438452
- Format
- –
- Schlagworte
- Acylation, Amino Acid Sequence, Amino acids, Arabidopsis - genetics, Arabidopsis - metabolism, Arabidopsis Proteins - chemistry, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Brefeldin A - pharmacology, Calcium, Calcium-Binding Proteins - chemistry, Calcium-Binding Proteins - genetics, Calcium-Binding Proteins - metabolism, Cell Membrane - metabolism, Cell membranes, Fatty Acids - chemistry, Fatty Acids - metabolism, Fatty Acids, Monounsaturated - chemistry, Fatty Acids, Monounsaturated - metabolism, Fluorescence, Gas Chromatography-Mass Spectrometry, Green Fluorescent Proteins - genetics, Green Fluorescent Proteins - metabolism, Lipids, Microscopy, Fluorescence, Molecular Sequence Data, Physiological regulation, Plant cells, Protein Synthesis Inhibitors - pharmacology, Protein Transport - drug effects, Protein-Serine-Threonine Kinases - genetics, Protein-Serine-Threonine Kinases - metabolism, Proteins, Sensors, Sequence Homology, Amino Acid, Signal Transduction - drug effects, Signal Transduction - physiology