Details

Autor(en) / Beteiligte

• Ohta, M
• Mimori, K
• Fukuyoshi, Y
• Kita, Y
• Motoyama, K
• Yamashita, K
• Ishii, H
• Inoue, H
• Mori, M

Titel

Clinical significance of the reduced expression of G protein gamma 7 ( ) in oesophageal cancer

Ist Teil von

• British journal of cancer, 2008-01, Vol.98 (2), p.410-417

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• We previously cloned human G protein gamma 7 ( GNG7 ) and demonstrated that it was downregulated in gastrointestinal cancer. The significance of GNG7 expression in oesophageal cancer is unknown. TaqMan quantitative real-time PCR was performed to determine the clinical significance of GNG7 expression in 55 cases of oesophageal cancer. Furthermore, GNG7 -transfected oesophageal cancer cells were analysed in laboratory studies at genomic and epigenetic levels. Twenty-seven patients with low GNG7 expression showed significantly poorer survival than did 28 patients with high expression ( P <0.05). Tumours with low GNG7 expression invaded deeper than those with high GNG7 expression ( P <0.05), both in vivo and in vitro . Eight tumours retained GNG7 expression, and they did not show either promoter hypermethylation or loss of heterozygosity (LOH). In 38 tumours with GNG7 suppression, 22 (57%) showed either LOH or promoter hypermethylation. In addition, GNG7 expression was significantly associated with the presence of miR328 in oesophageal cancer cell lines, which suggests that this microRNA might be a regulator of GNG7 expression. GNG7 suppression represents a new prognostic indicator in cases of oesophageal cancer. GNG7 might be suppressed by LOH and promoter hypermethylation or by microRNA.