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CA1 pyramidal to basket and bistratified cell EPSPs: dual intracellular recordings in rat hippocampal slices
Ist Teil von
The Journal of physiology, 1998-02, Vol.507 (1), p.201-217
Ort / Verlag
Oxford, UK: The Physiological Society
Erscheinungsjahr
1998
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Dual intracellular recordings in the CA1 region of adult rat hippocampal slices and biocytin filling of synaptically connected
cells were used to study the excitatory postsynaptic potentials (EPSPs) elicited in basket ( n = 7) and bistratified interneurones ( n = 7) by action potentials activated in simultaneously recorded pyramidal cells.
Interneurones could be subdivided according to their electrophysiological properties into classical fast spiking, burst firing,
regular spiking and fast spiking cells with a rounded spike after-hyperpolarization. These physiological classes did not,
however, correlate with morphological type. EPSPs were not recorded in regular spiking cells.
Average EPSP amplitudes were larger in bistratified cells (range, 0.5â9 mV) than in basket cells (range, 0.15â3.6 mV) and
the probability of obtaining a pyramidal cell-interneurone EPSP was also higher for the bistratified cells (1:7) than for
the basket cells (1:22). EPSP 10â90% rise times in bistratified cells (0.7â2 ms) and their widths at half-amplitude (3.9â11.2
ms) were slightly longer than in basket cells (rise times, 0.4â1.6 ms; half-widths, 2.2â9.7 ms).
The majority of these EPSPs (6 of 8 tested) increased in amplitude and duration with postsynaptic depolarization, although
in two (of 4) basket cells the voltage relation was conventional.
All EPSPs tested in both basket ( n = 7) and bistratified cells ( n = 5) decreased in amplitude with repetitive presynaptic firing. The average amplitudes of second EPSPs elicited within 15
ms of the first were between 34 and 94% of the average amplitude of the first EPSP. Third and fourth EPSPs in brief trains
were further depressed. This depression was associated with an increase in the incidence of apparent failures of transmission
indicating a presynaptic locus.