Details

Autor(en) / Beteiligte

• Dubois, B
• Vanbervliet, B
• Fayette, J
• Massacrier, C
• Van Kooten, C
• Brière, F
• Banchereau, J
• Caux, C

Titel

Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes

Ist Teil von

• The Journal of experimental medicine, 1997-03, Vol.185 (5), p.941-952

Ort / Verlag

United States: The Rockefeller University Press

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• After antigen capture, dendritic cells (DC) migrate into T cell-rich areas of secondary lymphoid organs, where they induce T cell activation, that subsequently drives B cell activation. Here, we investigate whether DC, generated in vitro, can directly modulate B cell responses, using CD40L-transfected L cells as surrogate activated T cells. DC, through the production of soluble mediators, stimulated by 3- to 6-fold the proliferation and subsequent recovery of B cells. Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells). In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM. Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.