Details

Autor(en) / Beteiligte

• Ceredig, R
• Jenkinson, E J
• MacDonald, H R
• Owen, J J

Titel

Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments

Ist Teil von

• The Journal of experimental medicine, 1982-02, Vol.155 (2), p.617-622

Ort / Verlag

United States: The Rockefeller University Press

Erscheinungsjahr

1982

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The appearance of immunologically competent cells in the organ-cultured mouse fetal thymic rudiment has been investigated. Fetal thymuses removed at 14 d of gestation and cultured for 7-21 d were assayed for their content of cytolytic T lymphocyte precursors (CTL-P) directed against H-2d alloantigens. Whereas CTL-P were undetectable within fetal thymus until 18-19 d of gestation, their frequency in the organ-cultured fetal thymus was similar to, or greater than that found in the normal adult thymus. This direct demonstration of the appearance of alloreactive CTL-P in a closed in vitro system should provide an accessible model for the investigation of interactions between developing T cells and the thymic microenvironment.