The Journal of cell biology, 2004-09, Vol.166 (7), p.969-974
2004
Details
- Autor(en) / Beteiligte
- Titel
- An AIF Orthologue Regulates Apoptosis in Yeast
- Ist Teil von
- The Journal of cell biology, 2004-09, Vol.166 (7), p.969-974
- Ort / Verlag
- United States: Rockefeller University Press
- Erscheinungsjahr
- 2004
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek
- Beschreibungen/Notizen
- Apoptosis-inducing factor (AIF), a key regulator of cell death, is essential for normal mammalian development and participates in pathological apoptosis. The proapoptotic nature of AIF and its mode of action are controversial. Here, we show that the yeast AIF homologue Ynr074cp controls yeast apoptosis. Similar to mammalian AIF, Ynr074cp is located in mitochondria and translocates to the nucleus of yeast cells in response to apoptotic stimuli. Purified Ynr074cp degrades yeast nuclei and plasmid DNA. YNR074C disruption rescues yeast cells from oxygen stress and delays age-induced apoptosis. Conversely, overexpression of Ynr074cp strongly stimulates apoptotic cell death induced by hydrogen peroxide and this effect is attenuated by disruption of cyclophilin A or the yeast caspase YCA1. We conclude that Ynr074cp is a cell death effector in yeast and rename it AIF-1 (Aif1p, gene AIF1).
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9525eISSN: 1540-8140DOI: 10.1083/jcb.200404138Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2172025
- Format
- –
- Schlagworte
- Aging, Apoptosis, Apoptosis - genetics, Apoptosis Inducing Factor, Caspase Inhibitors, Caspases - metabolism, Cell cycle, Cell death, Cell nucleus, Cell Nucleus - genetics, Cell Nucleus - metabolism, Cells, Cells, Cultured, Cellular biology, Cellular Senescence - genetics, Cyclophilin A - antagonists & inhibitors, Cyclophilin A - metabolism, Directional control, DNA, DNA - genetics, DNA - metabolism, DNA, Complementary - analysis, DNA, Complementary - genetics, Enzyme Inhibitors - pharmacology, Flavoproteins - genetics, Flavoproteins - isolation & purification, Flavoproteins - metabolism, Hydrogen Peroxide - pharmacology, Mammals, Membrane Proteins - genetics, Membrane Proteins - isolation & purification, Membrane Proteins - metabolism, Mitochondria, Mitochondria - genetics, Mitochondria - metabolism, Molecular Sequence Data, Mother cells, NADH, NADPH Oxidoreductases - genetics, NADH, NADPH Oxidoreductases - isolation & purification, NADH, NADPH Oxidoreductases - metabolism, Oxidative Stress - genetics, Plasmids, Protein Transport - genetics, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - antagonists & inhibitors, Saccharomyces cerevisiae Proteins - genetics, Saccharomyces cerevisiae Proteins - isolation & purification, Saccharomyces cerevisiae Proteins - metabolism, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Yeast, Yeasts