Details

Autor(en) / Beteiligte

• Jorgensen, A. O.
• A. C.-Y. Shen
• Campbell, K. P.
• MacLennan, D. H.

Titel

Ultrastructural Localization of Calsequestrin in Rat Skeletal Muscle by Immunoferritin Labeling of Ultrathin Frozen Sections

Ist Teil von

• The Journal of cell biology, 1983-11, Vol.97 (5), p.1573-1581

Ort / Verlag

New York, NY: Rockefeller University Press

Erscheinungsjahr

1983

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• The ultrastructural localization of calsequestrin in rat skeletal muscle (gracilis) was determined by indirect immunoferritin labeling of ultrathin frozen sections. Calsequestrin was found in the lumen of the transversely and longitudinally oriented terminal cisternae but was absent from most of the longitudinal sarcotubules and the fenestrated sarcoplasmic reticulum. Calsequestrin was occasionally observed in vesicular structures found in the central region of the I band. Since calsequestrin is believed to provide the major site of Ca2+sequestration in the sarcoplasmic reticulum, the present results support the view that Ca2+, transported to the lumen of the sarcoplasmic reticulum, is preferentially sequestered in the terminal cisternae, but they also suggest that additional Ca2+sequestration may occur near the center of the I band.